全氟辛烷磺酸和全氟辛酸对秀丽隐杆线虫的生殖毒性

doi:10.3969/j.issn.1004-616x.2016.06.015

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2016, Vol. 28 ›› Issue (6): 484-486,493.doi: 10.3969/j.issn.1004-616x.2016.06.015

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Reproductive toxicity of perfluorooctane sulfonate and perfluorooctanoic acid on Caenorhabditis elegans

JIAN Zihai, DAI Shuhao, WANG Xuefei, YANG Zijie, LU Qiang, YANG Zhenglong, AWUYING·Hadelebieke, YIN Lihong, LIU Ran

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China

Received:2016-08-28 Revised:2016-10-13 Online:2016-11-30 Published:2016-11-30

Abstract

Abstract: OBJECTIVE: Caenorhabditis elegans were used to investigate the induction of reproductive toxicity of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA),as an alternative models for evaluating reproductive toxicity. METHODS: Three PFOS/PFOA dose (0.1,0.01,0.001 mmol/L) groups and a control group were organized. After synchronization,L4 larvae were exposed for 24 h in 24-well plates,the L1 larvae were exposed for 48 h. The brood size and the generation time of Caenorhabditis elegans were evaluated to determine the induction of reproductive toxicity by PFOS and PFOA. RESULTS: Compared with the control,the high and the medium dose groups with exposure for 24 h showed significant difference in the brood size (P<0.05),and the three PFOA exposure dose groups showed significant difference among them (P<0.05). Generation time showed no significant difference among the exposed and the control groups. Compared with the control group,the high and medium dose groups with exposure for 48 h showed significant difference in the brood size (P<0.05) and the three exposed groups showed significant difference in generation time (P<0.05). CONCLUSION: The results suggested that brood size of Caenorhabditis elegans was a sensitive indicator for evaluating reproductive toxicity of PFOS and PFOA. In addition,generation time measurements was more sensitive using L1 larvae than that of L4 larvae.

Key words: PFOS, PFOA, Caenorhabditis elegans, brood size, generation time

CLC Number:

R994.6

JIAN Zihai, DAI Shuhao, WANG Xuefei, YANG Zijie, LU Qiang, YANG Zhenglong, AWUYING·Hadelebieke, YIN Lihong, LIU Ran. Reproductive toxicity of perfluorooctane sulfonate and perfluorooctanoic acid on Caenorhabditis elegans[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 484-486,493.

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Reproductive toxicity of perfluorooctane sulfonate and perfluorooctanoic acid on Caenorhabditis elegans

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