Carcinogenesis, Teratogenesis & Mutagenesis ›› 2016, Vol. 28 ›› Issue (6): 468-471.doi: 10.3969/j.issn.1004-616x.2016.06.011

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Differentially expressed miRNA profile in workers with trichloroethylene-induced medicamentosa-like dermatitis

ZHENG Jian1,2, LAN Tao2, LIU Wei2, DENG Rongxia2, LI Peimao3, ZHANG Yanfang3, LIU Jianjun1,2   

  1. 1. Department of Preventive Medicine, Medicine School of Hunan Normal University, Changsha 410013, Hunan;
    2. Shenzhen Center for Disease Control & Prevention, Shenzhen 518055, Guangdong;
    3. Shenzhen Prevention and Treatment Center for Occupational Disease, Shenzhen 518001, Guangdong, China
  • Received:2016-07-12 Revised:2016-11-01 Online:2016-11-30 Published:2016-11-30

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Abstract: OBJECTIVE: To profile microRNAs expression among workers with trichloroethylene (TCE)-induced medicamentosa-like dermatitis (OMLDT). METHODS: Serum samples from 5 patients with OMLDT and with TCE contacts were collected. The total RNA was extracted then labeled with Hy3 fluorescent. MicroRNAs were hybridized with miRCURY LNA chips and images were acquired using the GenePix 4000B scanner,qPCR method was used to verify the results of miRNA microarray. The results were further analyzed by prediction softwares. RESULTS: A total of 69 microRNAs were differentially expressed between OMLDT and TCE contacts. Among the microRNAs,39 were up-regulated and 30 were down-regulated. Increase of miR-199b-5p was verified using qPCR. Target prediction results showed that the same target genes of miR-409-3p and miR-485-3p had been predicted in all forecasting software. CONCLUSION: The study showed that expression of microRNA in serum of workers with OMLDT was significantly altered,and miR-199b might serve as a candidate biomarker for OMLDT.

Key words: miRNA, occupational medicamentosa-like dermatitis induced by trichloroethylene, target gene prediction, biomarkers

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