应用Ames波动试验比较4种马兜铃酸组分的致突变作用

doi:10.3969/j.issn.1004-616x.2016.05.013

Abstract

Abstract: OBJECTIVE: To compare the mutagenicity among four components of aristolochic acid (AA-I,AA-Ⅱ,AA-Ⅲ and AA-IV) using the Ames fluctuation test,and to evaluate the relationship between toxicity mechanisms and molecular structures of aristolochic acid. METHODS: Two Salmonella typhimurium tester strains (TA98 and TA100) were employed to perform the Ames fluctuation test in the absence and presence of the S9 exogenous metabolic activation system. RESULTS: The mean number of revertant wells of AA-I and AA-Ⅱ were significantly increased in tester strains TA98 and/or TA100,with and without S9 (P<0.05). AA-Ⅱ showed a clear dose-response increase and the fold inductions in mean number of revertant wells over the baseline of AA-Ⅱ and more than AA-I. Both AA-Ⅲ and AA-IV did not induce significant increase with the two test strains,but AA-IV showed a trend of dose-response increase. CONCLUSION: AA-I and AA-Ⅱ were mutagenic with or without metabolic activation,and the mutagenicity of AA-Ⅱ was stronger than that of AA-I. AA-IV demonstrated mutagenicity with metabolic activation. AA-Ⅲ did not show mutagenicity in the two tester strains. The four aristolochic acids exhibited different levels of mutagenicity which might be due to their different structural and metabolic characteristics.

Key words: aristolochic acid, Ames fluctuation test, genotoxicity, mutagenicity

CLC Number:

R394.6

CAO Yiyi, LIU Qian, XI Jing, MA Leilei, LIU Xiaojian, LUAN Yang. Comparative evaluation of mutagenicity of four aristolochic acids components using the Ames fluctuation test[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 398-402.

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Comparative evaluation of mutagenicity of four aristolochic acids components using the Ames fluctuation test

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