红景天苷对氯气暴露致急性肺损伤肺血管通透性的保护作用

doi:10.3969/j.issn.1004-616x.2016.05.009

Abstract

Abstract: OBJECTIVE: To investigate the protective effects of salidroside on acute lung injury (ALI) in rats which were exposed to chlorine,and to explore the protective mechanism. METHODS: Fortymale Sprague-Dawley (SD) rats were randomly divided into four groups:control,chlorine,chlorine+salidroside and salidroside. Chlorine and chlorine+salidroside groups were exposed to chlorine of 1 200 mg/m³ for 5 minutes. Chlorine+salidroside and salidroside groups were treated with salidroside (300 mg/kg) at 30 minutes before chlorineexposure and at 15 minutes after chlorine exposure. At 3 hours after chlorine exposure,histopathological analysis was conducted to evaluate the degree of ALI and dihydroethidium (DHE) assay was used to determine the level of pulmonary reactive oxygen species (ROS). Lung permeation index was calculated by protein detecting. The malondialdehyde (MDA),superoxide dismutase (SOD),L-glutathione (GSH) and oxidized glutathione (GSSG) in BALF were measured by kits. The protein level of SOD1 and SOD2 were detected by western blotting. RESULTS: At 3 hours after chlorine exposure,there appeared marked histopathological changes in the lung,including macrophage and neutrophile granulocyte infiltration and edema emerging in bronchiolar and pulmonary alveolar. The ROS level in lung and the lung permeation index were higher. MDA, SOD,GSSG of BALF increased significantly (P<0.05) compared with control group. The GSH and GSH/GSSG ratio in BALF were lower than that in the control group (P<0.05). SOD activity in BALF,and SOD1 and SOD2 protein expression were significantly elevated in chlorine group. In the chlorine+salidroside group,the lung permeation index,ROS level in the lung,MDA and GSSG in BALF decreased significantly (P<0.05) compared with the chlorine group. The GSH/GSSG ratio in BALF were higher than that in the chlorine group (P<0.05). SOD1 and SOD2 protein expression were significantly reduced (P<0.05). CONCLUSION: Our data show that chlorine exposure induced ALI. Administration of salidroside reduce the chlorine-induced ALI via reduction of oxidative stress.

Key words: chlorine, oxidative stress, salidroside, acute lung injury, antioxidation

CLC Number:

R114

LIU Mengmeng, KONG Deqin, ZOU Yuankang, WANG Lele, LIU Rui, HAI Chunxu, ZHANG Xiaodi. Protective effects of salidroside on chlorine-induced acute lung injury in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 377-382.

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Protective effects of salidroside on chlorine-induced acute lung injury in rats

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