婆罗双树样基因2干扰对宫颈癌HeLa细胞增殖和凋亡的影响

doi:10.3969/j.issn.1004-616x.2016.05.005

Abstract

Abstract: OBJECTIVE: To investigate the interference of sal-like 2 (SALL2) gene on proliferation and apoptosis of the cervical carcinoma HeLa cells. METHODS: 3 small interference RNA (siRNA-1,2,3) which targeted SALL2 were structured,and a carrier group and a control group were set. Liposome was used to transfect SALL2-siRNA into HeLa cells. Expression of SALL2 mRNA and protein in the transfected cells were detected by the method of RT-PCR and Western blot by screening the siRNA fragment with the best silencing effect. After the most suitable siRNA fragment was transfected into HeLa cells,CCK-8 was used to detect the cell proliferation rate,and flow cytometry was used to test cell cycle and apoptosis rates. RESULTS: SALL2 was highly expressed in HeLa cells of the control group. Compared with the control group,SALL2 mRNA and protein expression in the carrier group and the siRNA-3 transfection group showed no statistically significant differences (P>0.05),while those in HeLa cells were significantly reduced after transfected with siRNA-1 and siRNA-2 for 48 h (P<0.05). Among them,siRNA-1 showed the best silencing effect,thus siRNA-1 was subsequently selected as the interference group. In contrast with the control group,cell proliferation rate increased significantly after the transfection with siRNA-1 for 48 and 72 h (P<0.05);the proportion of cells in the G0/G1 phase decreased dominantly and apoptosis rate reduced significantly in the siRNA-1 group after transfection for 48 h (P<0.05). CONCLUSION: SALL2 gene interference improved the proliferation rate and reduced the apoptosis rate in HeLa cells,suggesting that SALL2 gene served as a tumor suppressor gene in cervical carcinoma HeLa cells.

Key words: cervical carcinoma, SALL2 gene, cell proliferation, cell cycle, apoptosis

CLC Number:

R392.1

ZHANG Xueshan, MIAO Fang, CAO Yanning, ZHANG Xiaoshu. SALL2 interference on proliferation and apoptosis of cervical carcinoma HeLa cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 359-363,368.

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SALL2 interference on proliferation and apoptosis of cervical carcinoma HeLa cells

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