Carcinogenesis, Teratogenesis & Mutagenesis ›› 2016, Vol. 28 ›› Issue (5): 353-358,392.doi: 10.3969/j.issn.1004-616x.2016.05.004

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Differential expression of Gadd45α in Helicobacter Pylori-infected human gastric cancer and clinical significance

FENG Shuitu1, CHEN Yide1, CHEN Yuqiang2, LIN Yubiao1, ZHENG Wenhui1   

  1. 1. Department of Medical Oncology, Xiamen Haicang Hospital, Xiamen 361026;
    2. Clinical Center of Tumor Therapy, Affiliated Chenggong Hospital of Xiamen University/the 174th Hospital of PLA, Xiamen 361004, Fujian, China
  • Received:2015-12-14 Revised:2016-06-13 Online:2016-09-30 Published:2016-09-30

Abstract: OBJECTIVE: To investigate the differential expression level of Gadd45α in human gastric cancer with Helicobacter Pylori (HP) infection status and the clinical significance of the expression for improvedrclinical diagnosis and treatment for gastric cancer. METHODS: 70 paraffin-embeded samples of gastric cancerwith HP infection status and 56 normal tissues from surgically resected tissues were selected at random. The expressions of Gadd45α protein in the samples were detected by immuno-histochemical staining. The relationship between the positive expression of Gadd45α protein and the clinico-pathological parameters of gastric cancer was analyzed. Furthermore,expression levels of Gadd45α mRNA and protein in 8 fresh gastric cancer specimens were detected using Western blot and RT-PCR. RESULTS: Immuno-histochemical analyses showed that the positive expression rate of Gadd45α protein in HP+ gastric carcinoma tissues,HP- gastric carcinoma tissues and HP+ adjacent non-neoplastic tissues were 77.5%,46.67% and 34.21%,respectively. Their differences were significant (P<0.05). The expression rate in gastric carcinoma tissues was 64.29% and it was correlated with tumor size. The expression of Gadd45α protein in gastric carcinoma was positively correlated with HP infection status (rs=0.318,P<0.05) by a correlated statistical analysis of Spearman's Rho. Western blot and RT-PCR detection results showed that HP+gastric cancer tissues had a higher expression in protein and mRNA levels of Gadd45α in fresh specimens than in adjacent non-neoplastic tissues. CONCLUSION: Expression of Gadd45α protein was up-regulated,especially in HP+ gastric carcinomas. The expression level was closely related to the occurrence and development of gastric carcinoma (especially HP+). The detection of the expression of Gadd45α protein was helpful for early diagnosis and for prediction of prognosis of gastric carcinoma. The expression of Gadd45α protein and Helicobacter Pylori infection status in gastric carcinoma were positively correlated. This was probably correlated with the development of gastric carcinoma. The prognosis of HP+gastric carcinoma might be affected by anti HP treatment.

Key words: gastric cancer, Gadd45α, Helicobacter Pylori, immunohistochemistry

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