TGF-β1在黑色素瘤血清中的异常表达及其对肿瘤细胞凋亡的影响

doi:10.3969/j.issn.1004-616x.2016.05.003

Abstract

Abstract: OBJECTIVE: Melanoma usually has poor prognosis,thus the aims of this study were to find potential targets of melanoma in serum and to understand their roles in the progression of melanoma. METHODS: Serum samples from patients and healthy controls were collected. From the samples,IgG and albumin were removed,and proteins were digested and labeled with iTRAQ labeling reagent. Differentially expressed serum proteins were identified by LC-MS/MS and validated by ELISA. TGF-β1 overexpressed melanoma cells were constructed using PLVX plasmid and lentivirus transfection. Cell apoptosis were analyzed by flow cytomeric assay. RESULTS: 81 melanoma-associated proteins were identified,33 were up-regulated and 49 were down-regulated. TGF-β1 was found significantly down-regulated (3.17±0.76,P<0.05) in melanoma patients' serum and validated by ELISA. TGF-β1 overexpressed melanoma cells (A-375 and SK-MEL-1) were constructed,the apoptosis analysis showed that TGF-β1 could significantly induced apoptosis of melanoma cells. CONCLUSION: TGF-β1 was reduced in melanoma patients' serum and was involved with inhibition of tumor cell proliferation. Thus,the findings suggest that TGF-β1 could be an important potential target in melanoma.

Key words: melanoma, proteomics, TGF-β1, cell apoptosis

CLC Number:

R730.231

ZHOU Qiming, LI Wei, YUAN Jianhui, ZHAO Yan, DUAN Jiangman, XIONG Donglin. Abnormal expression of TGF-β1 in melanoma patients and its inhibitory effect on tumor cell proliferation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 348-352.

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Abnormal expression of TGF-β1 in melanoma patients and its inhibitory effect on tumor cell proliferation

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