采用高效液相色谱法检测余甘子中的没食子酸

doi:10.3969/j.issn.1004-616x.2016.04.013

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2016, Vol. 28 ›› Issue (4): 310-313,328.doi: 10.3969/j.issn.1004-616x.2016.04.013

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Determination of gallic acid in Phyllanthus emblica L. by high performance liquid chromatography

ZHENG Yilin¹, ZHENG Fuchun², LI Chunquan³, LI Miaoxia³, CHEN Chaosheng³, YE Danyan¹, CHEN Peihong¹, CHEN Yicun¹

1. Department of Pharmacology, Shantou University Medical College, Shantou 515041;
2. Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College, Shantou 515041;
3. Guangdong Huiqun Chinese Herbal Medicine Co., Ltd., Shantou 515041, Guangdong, China

Received:2015-09-18 Revised:2016-05-24 Online:2016-07-31 Published:2016-07-31

Abstract

Abstract: OBJECTIVE: To optimize chromatographic conditions for determination of gallic acid contents in Phyllanthus emblica L. from different regions. METHODS: Determination of gallic acid in Phyllanthus emblica L. in different regions was performed on an Eclipse plus C18 (25 μm, 4.6 mm×250 mm). The mobile phase was Acetonitrile-0.2% phosphoric acid solution (5:95). The elution was in same degree. The current speed was 1.000 mL/min. The detection wave length was 273 nm and the determination of linear regression equation used standard curve method. The column temperature was 30 degrees Celsius. RESULTS: Gallic acid had a good linear relationship in the range of 13.010-130.104 g/mL (R²=0.999 4). It also had good precision (RSD=0.08%), good stability (RSD=0.29%) and general reproducibility (RSD=4.23%). The content of gallic acid in the three batches of Phyllanthus emblica L. were higher than Chinese Pharmacopoeia national standard of 1.2%. CONCLUSION: The method for the determination of gallic acid in Phyllanthus emblica showed strong specificity and good precision. In addition, the results were accurate and reliable.

Key words: high performance liquid chromatography, Phyllanthus emblica L., gallic acid, determination of content

CLC Number:

R917

ZHENG Yilin, ZHENG Fuchun, LI Chunquan, LI Miaoxia, CHEN Chaosheng, YE Danyan, CHEN Peihong, CHEN Yicun. Determination of gallic acid in Phyllanthus emblica L. by high performance liquid chromatography[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 310-313,328.

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Determination of gallic acid in Phyllanthus emblica L. by high performance liquid chromatography

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