冈田酸对喉鳞癌细胞系PP2A活性及迁移的影响

doi:10.3969/j.issn.1004-616x.2016.04.005

Abstract

Abstract: OBJECTIVE: To investigate the mechanism of inhibition of Hep-2 cell migration by Okadaic acid (OA). METHODS: MTT assay was used to determine the doses of OA for the investigation. Hep-2 cells were treated with 0, 50 or 100 nmol/L OA and enzyme assay was used to measure PP2A activities. Wound scratch assay was used to observe the influence of OA on migration of Hep-2. RESULTS: The growth inhibition ratio was 96.7%±1.8%, 82.9%±12.6% and 57.2%±7.7% in cells treated with 50, 100 and 200 nmol/L OA, and the difference was significant (P<0.05). In cells treated with 50 and 100 nmol/L OA, the relative activity of PP2A was 30.90%±12.01% and 8.98%±4.96% (P<0.05). From the wound scratch assay, the treated cells took significantly longer than untreated cells for healing (P<0.05). CONCLUSION: Inhibition of PP2A activities may be responsible for the reduction of migration in the OA-treated Hep-2 cells.

Key words: Okadaic acid, Hep-2, laryngeal squamous cell carcinoma, protein phosphatase 2A, migration

CLC Number:

R73-3

TONG Yusheng, CHEN Tao, WANG Conghui, YUAN Hui, XU Lihong, LIN Zhihong. Okadaic acid inhibits PP2A activities and cell migration of laryngeal squamous cell carcinoma Hep-2[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 273-276,280.

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Okadaic acid inhibits PP2A activities and cell migration of laryngeal squamous cell carcinoma Hep-2

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