不同诱导方法制备大鼠肝微粒体酶及其活性的比较

doi:10.3969/j.issn.1004-616x.2016.04.004

Abstract

Abstract: OBJECTIVE: To compare different induction methods on activity of rat liver microsomal enzyme S9 and to identify the effective induction method. METHODS: Rats were injected with PCBs, phenobarbital, beta- naphthoflavone and phenobarbitol sodium. In addition, beta-naphthoflavone and phenobarbital were used as inducer to induce rat liver S9. S9 protein content was determined by the method of Lowry. Metabolic activation effects of four kinds of S9 were compared using the Ames test and the in vitro chromosome aberration assay of CHL cells. RESULTS: Protein contents of four kinds of S9 were 30.29, 34.15, 33.31, 28.02 mg/mL, all of them were less than 40 mg/mL. Compared with the groups without S9, the 4 types of S9 showed activation effect in the positive control (P<0.01). Activation effects from three kinds of S9 had no significant difference among them in the Ames and CHL chromosome aberration assays (P>0.05), however, the activation from the phenobarbital-induced S9 was significantly weaker (P<0.05 or P<0.01). CONCLUSION: Under our conditions:using PCBs, phenobarbital and beta-naphthoflavone, phenobarbitol sodium injection and beta-naphthoflavonen as inducer, metabolically active rat liver S9 was induced successfully. In addition, the combined induction method can take the place of the induction by PCBs alone.

Key words: liver microsomal enzymes, protein content, activation, Ames test, chromosome aberration assay

CLC Number:

R965

GAO Mei, CAO Chong, YING Yong, MA Hui, JIA Qingwen. Comparison of different inductionmethods on activity of rat liver microsomal enzymes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 269-272.

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Comparison of different inductionmethods on activity of rat liver microsomal enzymes

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