塞来昔布对T细胞淋巴瘤细胞增殖及侵袭功能的影响

doi:10.3969/j.issn.1004-616x.2016.04.002

Abstract

Abstract: OBJECTIVE: To investigate the effect of celecoxib on proliferation, chemotherapy sensitivity and invasion of T cell lymphoma. METHODS: Jurkat and Hut78 cells were treated with celecoxib for 24, 48 and 72 h, and their proliferation activity and chemotherapy sensitivity were detected using the MTT method. In addition, apoptotic rates of the treated cells were analyzed using flow cytometry. Transwell chamber was used to investigate the invasion of cells which were treated with celecoxib for 48 h. Expression of P38, P65, Bcl-2, Bax, MDR1, MMP2 and MMP9 in treated cells were investigated using quantitative real-time PCR (qPCR) and Western blot. RESULTS: After treatment with celecoxib, growth of Jurkat and Hut78 cells was inhibited in a dose-and time-dependent manner, and their chemotherapy sensitivity was elevated. Consequently, the apoptotic rates of the treated cells were also higher than the control. The number of treated cells that passed through Transwell chamber was significantly less than control (P<0.01). The qPCR and Western blot analyses showed that expression of P65, Bax, MDR1, Bcl-2, MMP2 and MMP9 were all down-regulated, whereas P38 and Bax were up-regulated in the treated cells compared with control(P<0.05). CONCLUSION: Celecoxib has inhibitory effect on proliferation and invasion of T cell lymphoma. Therefore, it may have a broad prospect for clinical treatment of T cell lymphoma.

Key words: celecoxib, T cell lymphoma, proliferation, invasion

CLC Number:

R733.7

MA Ming, YANG Xingxiao, WANG Xuexiao, LIU Lihua, ZHAO Lianmei, DU Yanyan, SHAN Baoen. Effects of celecoxib on proliferation and invasion of T cell lymphoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 255-261.

References

[1] 李杰,薛丽英,王超,等. 塞来昔布对NB4细胞增殖凋亡及VEGF表达的影响[J]. 肿瘤防治杂志,2013,40(2):147-150.
[2] Rudner J,Elsaesser SJ,Jendrossek V,et al. Anti-apoptotic Bcl-2 fails to form efficient complexes with pro-apoptotic Bak to protect from Celecoxib-induced apoptosis[J].Biochem Pharmacol,2011,81(1):32-42.
[3] 张德庆,祝建红,陈卫昌. 塞来昔布联合5-FU抑制裸鼠结肠癌生长及其机制的探讨[J]. 中华肿瘤防治杂志,2013,20(1):15-20.
[4] 赵连梅,韩丽娜,商晓辉,等. 木鳖子醇提物对黑素瘤B16细胞增殖的抑制及其可能机制[J]. 中国肿瘤生物治疗杂志,2010,17(1):13-18.
[5] 王玲,李杰,张璟,等. 塞来昔布对人三阴性乳腺癌裸鼠体内白细胞介素6和白细胞介素8水平的影响[J]. 中国医科大学学报,2012,41(9):801-804.
[6] 范立侨,李勇,李巧霞,等. 塞来昔布联合氟尿嘧啶对MFC细胞615小鼠原位移植瘤COX2、Fas表达的影响及意义[J]. 肿瘤学杂志,2015,21(5):355-359.
[7] Brown MD,Sacks DB. Protein scaffolds in MAP kinase signalling[J]. Cell Signal,2009,21(4):462-469.
[8] 高克非,冯艳玲,黄永文,等. 三氧化二砷联合塞来昔布对卵巢癌细胞的增殖抑制和凋亡诱导作用[J]. 肿瘤,2014,34(7):602-608.
[9] Sareddy GR,Geeviman K,Ramulu C,et al. The nonsteroidal anti-inflammatory drug celecoxib suppresses the growth and induces apoptosis of human glioblastoma cells via the NF-kappaB pathway[J]. J Neurooncol,2012,106(1):99-109.
[10] 姚娟,翁亚光,严树涓,等. 肾母细胞瘤过表达基因对人骨肉瘤143B细胞增殖、凋亡和迁移的影响[J]. 肿瘤,2015,35(2):119-128.
[11] Cui XB,PangXL,Li S,et al. Elevated expression patterns and tight correlation of the PLCE1 and NF-κB signaling in Kazakh patients with esophageal carcinoma[J]. Med Oncol,2014,31(1):791.
[12] 杨雪,欧俐苹,王晓荣,等. PLC基因通过NF-B/p65途径抑制肾癌786-0细胞的增殖[J]. 第三军医大学学报,2014,36(19):2010-2015.
[13] Bo Liang,Xiao-Ling Guo,Jing Jin,et al. Glycyrrhizic acid inhibits apoptosis and fibrosis in carbon tetrachloride-induced rat liver injury[J]. World J Gastroenterol,2015,21(17):5271-5280.
[14] Ma B,Zhou PY,Ni W,et al. Inhibition of activin receptor-like kinase 5 induces matrix metallopeptidase9 expression and aggravates lipopolysaccharide-induced pulmonary injury in mice[J]. Eur Rev Med Pharmacol Sci,2013,17(8):1051-1059.
[15] Kao SJ,Su JL,Chen CK,et al. Osthole inhibits the invasive ability of human lung adenocarcinoma cells via suppression of NF-κB mediated matrix metallo proteinase-9 expression[J]. Toxicol Appl Pharmacol,2012,261(1):105-115.

Effects of celecoxib on proliferation and invasion of T cell lymphoma

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Yan, SUN Zhenxiao. Metformin inhibitd proliferation and migration of human breast cancer cell in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 35-39.
[2]	WANG Xiaozhou, LI Hongshi, YU Xi, WEI Ning, WANG Ziying, HE Lijie. Effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 442-445.
[3]	YANG Xingxiao, ZHANG Xueyuan, ZOU Naiyi, SHAN Bao'en, MA Ming, ZHU Shuchai. Effects of HMGB1 silencing on migration, invasion and radiosensitivity of esophageal squamous cell carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 327-333.
[4]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[5]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[6]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[7]	FANG Lei, YANG Tao, SHENG Lei, MUTALIFU·Aimaiti, QI Xinxin, AINIWAER·Aikemu, YILIYAER·Nijiati. Biological effects of saponins from the seeds of Nigella glandulifera Freyn on human cervical cancer SiHa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 143-148,152.
[8]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.
[9]	LIN Kaihuang, CAI Gaoyang, LIU Xincheng. Effect of mTOR inhibitor AZD2014 on proliferation of hepatoma HCC cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 448-451.
[10]	LIU Peng, BAI Yixiao. Effect of miR-29a on proliferation and migration of human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 452-456,463.
[11]	CHEN Qi, ZHONG Qian, YUE Wentao. Inhibition of nasopharyngeal carcinoma proliferation by RBM24 via regulation of HOTAIR [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 275-280.
[12]	CHEN Mianling, MA Shuoyi. Regulation of migration and invasion of triple-negative breast cancer cell MDA-MB-231 by miR-21 via targeting PDCD4 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 292-297.
[13]	WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.
[14]	FANG Congwen, SHI Ying, DU Feng, KANG Bingwen, HAN Mingbo, YIN Caocao, BAI Hua, QIN Xujun. Involvement of high-mobility group box 1 in cellular proliferation during liver regeneration in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 81-86.
[15]	SHENG Lei, WU Guizhen, REYIMU Renaguli, AIKEMU Ainiwaer. Toxicological activities of total flavonoids from Xinjiang semen Nigellae in human cervical cancer cell lines [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 385-391.