纳米氧化硅对RAW264.7细胞的DNA损伤作用

doi:10.3969/j.issn.1004-616x.2016.04.001

Abstract

Abstract: OBJECTIVE: RAW264.7 cells were exposed to different doses ofsilica nanoparticles and micron silicon dioxide (SiO₂), and induction of DNA damage was investigated. METHODS: RAW264.7 cells were treated with 20 nm, 60 nm silica nanoparticles at dosage of 31.25-500 μg/mL and micron SiO₂ at dosage of 500 μg/mL for 24 h. DNA single-strand break was detected by the Comet single cell gel electrophoresis (SCGE) and all the indexes of the assay:rate of comet (r_comet), tail length (TL) of DNA, rate of tail DNA (r_{tail DNA}), and Olive tail moment (OTM) were analyzed by the Comet Assay Software Project (CASP). In addition, levels of malondialdehyde (MDA), hydroxyl radicals (^·OH), superoxide anion radical (O₂·), hydrogen peroxide (H₂O₂) and nitric oxide (NO) in homogenate supernatantfrom treatedcells were measured. RESULTS: r_comet, TL, r_{tail DNA} and OTM induced by microsized SiO₂ were significantly increased compared with the negative control at the concentration of 500 μg/mL (P<0.01). Cells exposed to 125 and 500 μg/mL of the two kinds of silica nanoparticles showed a distinct increase of r_comet, TL, r_{tail DNA} and OTM compared with the negative control (P<0.01). r_comet, r_{tail DNA} and OTM caused by microsized SiO₂ were significantly increased compared with the silica nanoparticles groups(P<0.01) at the same concentrations. The increase of lipid peroxidation products of MDA and free radicals of ^·OH, O₂·, H₂O₂ and NO were in different degrees the result from silica nanoparticle and micron SiO₂ in comparison to the negative control. CONCLUSION: Both silica nanoparticle and microsized SiO₂ can cause DNA single-strand break and oxidative injury in RAW264.7 cells. The generation of free radicals of oxidative stress may have played a role in induction of DNA-damage.

Key words: silica nanoparticle, RAW264.7 cells, free radical, DNA-damage, single cell gel electrophoresis

CLC Number:

Q355

YANG Hong, WU Qiuyun, LAO Canshan, LI Mingyue, LI Wenchao. DNA-damage in RAW264.7 cells exposed to different sizes of silica nanoparticles[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(4): 249-254.

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DNA-damage in RAW264.7 cells exposed to different sizes of silica nanoparticles

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