小窝蛋白-1和E-钙黏蛋白在食管癌TE1细胞系中的表达及其意义

doi:10.3969/j.issn.1004-616x.2016.03.014

Abstract

Abstract: OBJECTIVE: To detect the expression of Caveolin-1(Cav-1) and E-cadherin(E-cad) in esophageal carcinoma TE1 cell line and to explore the involvement of Cav-1 in metastasis of esophageal carcinoma. METHODS:Small interfering RNAs Cav-1 siRNA1 and Cav-1 siRNA2 which targeted Cav-1 and negative control siRNA were individually transfected into esophageal carcinoma TE1 cell line. Non-transfected cells were used as blank control. Expressions of Cav-1 and E-cad were detected in cell extracts using Western blot. Migration and invasion ability of cells were evaluated using the Transwell assay. RESULTS:The Western blot analysis showed that Cav-1 siRNAs effectively suppressed the expression of Cav-1 and up-regulated the expression of E-cad in TE1 cells. The transwell assay showed that the migration and invasion ability of Cav-1 siRNA treated group were significantly weakened compared with the blank control group (P<0.05). CONCLUSION:Cav-1 may affect metastasis of esophageal carcinoma by up-regulating the expression of E-cad.

Key words: caveolin-1, E-cadherin, esophageal carcinoma, TE1 cell line

CLC Number:

R735.1

CAI Xiaoman, CHEN Suzuan, YU Jing, LIU Zhaohui, WU Ruinuan, TANG Shengxin, GUO Guanghua. The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 227-230.

References

[1] Gupta R,Toufaily C,Annabi B. Caveolin and cavin family members:Dual roles in cancer[J]. Biochimie,2014,107:188-202.
[2] Li H,Chen Y,Yan D. Expression of caveolin-1 and MMP-2 in bladder cancer and its clinical significance[J]. Chin-Ger J Clin Oncol,2007,6(6):571-573.
[3] Tanase CP,Dima S,Mihai M,et al. Caveolin-1 overexpression correlates with tumour progression markers in pancreatic ductal adenocarcinoma[J]. J Mol Histol,2009,40(1):23-29.
[4] Gloushankova NA.Changes in regulation of cell-cell adhesion during tumor transformation[J]. Biochemistry:Moscow,2008,73(7):742-750.
[5] Zhai JW,Yang XG,Yang FS,et al. Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma[J]. Chin J Cancer,2010,29(3):317-320.
[6] Zhai J,Yang X,Zhang Y,et al. Reduced expression levels of the death-associated protein kinase and E-cadherin are correlated with the development of esophageal squamous cell carcinoma[J]. Exp Ther Med,2013,5(3):972-976.
[7] Bosch FX,Andl C,Abel U,et al. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma:a comparison of E-cadherin with desmosomal components[J]. IJC,2005,114(5):779-790.
[8] Alshenawy HA,Ali MA.Differential caveolin-1 expression in colon carcinoma and its relation to E-cadherin-β-catenin complex[J]. Ann Diagn Pathol,2013,17:476-482.
[9] Kato K,Hida Y,Miyamoto M,et al. Overexpression of Caveolin-1 in esophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage[J]. Am Cancer Soc,2002,94(4):929-933.
[10] Ando T,Ishiguro H,Kimura M,et al. The overexpression of caveolin-1 and caveolin-2 correlates with a poor prognosis and tumor progression in esophageal squamous cell carcinoma[J]. Oncol Rep,2007,18(3):601-609.
[11] 章昱,俞晶,刘朝晖,等. ROCK抑制剂对人食管癌EC9706细胞迁移能力的影响[J]. 中国临床研究,2014,27(2):129-131
[12] 吴瑞暖,陈素钻,俞晶,等. ROCK抑制剂对TE13细胞生长及迁移能力的影响[J]. 中国临床研究,2014,27(1):5-7
[13] Bailey KM,Liu J. Caveolin-1 up-regulation during epithelial to mesenchymal transition is mediated by focal adhesion kinase[J]. J Biol Chem,2008,283(20):13714-13724.

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The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1

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