原花青素对脂多糖诱导BV2小胶质细胞炎症介质分泌的影响

doi:10.3969/j.issn.1004-616x.2016.03.011

Abstract

Abstract: OBJECTIVE:To investigate the effects of pro-anthocyanidin on inhibiting inflammatory mediator secretion in LPS activation of BV2 microglia. METHODS:Neuroinflammation model was established by LPS-activated BV2 microglia.BV2 microglial were pretreated with different concentrations of pro-anthocyanidin (0.1, 0.5, 1.0, 5.0 μg/mL) and stimulated with LPS (1.0 μg/mL) for 24 h. Cell viability was assessed by MTT assay. Griess method was utilized to detect the content of NO. The secretion level of pro-inflammatory cytokines TNF-alpha, IL-1beta and IL-6 was examined using specific ELISA kits. RESULTS:Within a certain dosage ranges of pro-anthocyanidin and LPS, there was no negative effect on cell viability (P>0.05). LPS (1.0 μg/mL) significantly increased the release of inflammatory mediator nitric oxide (NO) as well as the cytokines TNF-α, IL-1β and IL-6(P<0.05). Compared with the LPS group, different concentrations of pro-anthocyanidin (0.1, 0.5, 1.0, 5.0 μg/mL) attenuated the release of NO, TNF-α, IL-1β and IL-6 in cells that were cultured in conditioned media from LPS-induced BV2 microglia. The effect was dose-dependent (P<0.05). CONCLUSION:We showed that pro-anthocyanidin has a protective effect on inflammatory responses of LPS-activated BV2 microglia in vitro.

Key words: proanthocyanidin, BV2 microglia, lipopolysaccharide, neuroinflammation

CLC Number:

R994.6

CHEN Jinzhi, ZHANG Xiaoqiang, QU Zhihua, ZHOU Yapan, ZHANG Yan, LIANG Xiaoyu. Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 214-217.

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Anti-inflammatory effects of proanthocyanidin on activation of BV2 microglia by lipopolysaccharide

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