Prohibitin在放射照射诱导鼻咽癌细胞凋亡中的表达及亚细胞定位的变化

doi:10.3969/j.issn.1004-616x.2016.03.008

Abstract

Abstract: OBJECTIVE:To investigate changes in expression and subcellular localization of prohibitin (PHB) in radiation-induced apoptosis in nasopharyngeal carcinoma cells. METHODS:CNE-1 and CNE-2 cells were cultured in vitro. Their rates of early stage apoptosis after 4 Gy radiation were measured by flow cytometry.Expression of PHB, Bax and P53 proteins were measured by Western blot. In addition, immunofluorescence was used to detect their intracellular locations. RESULTS:At 24 hours after radiation, the apoptosis rates and expression of PHB, Bax and P53 were increased significantly. Before radiation, both PHB and P53 were distributed mainly around the nucleus but much less in the cytoplasm. After radiation, the fluorescence intensity in the cytoplasmic region increased dramatically, while the intensity in the nucleus dropped. It seemed that nuclear export of PHB and P53 were induced by radiation. However, no significant subcellular localization could be found in Bax distribution. CONCLUSION:The radiation-induced apoptosis may be associated with the expression and positional alteration of PHB and P53. Furthermore, PHB and P53 may interact with each other.

Key words: nasopharyngeal carcinoma, prohibitin, radiation, subcellular localization

CLC Number:

R730.2

CHEN Jiongyu, PENG Lin, HUANG Yiteng, LIN Xueqiong. Involvement of prohibitin during radiation-induced apoptosis in nasopharyngeal carcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 200-204.

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Involvement of prohibitin during radiation-induced apoptosis in nasopharyngeal carcinoma cells

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