铅暴露工人外周血淋巴细胞遗传损伤与端粒长度的改变

doi:10.3969/j.issn.1004-616x.2016.03.004

Abstract

Abstract: OBJECTIVE:To investigate the induction of DNA damage and telomere length changes in peripheral leukocytes from lead exposed works in comparison with un-exposed controls.METHODS:21 lead exposed workers from a battery plant in the Pearl River Delta Region and 26 non-exposed workers were investigated. Questionnaires were surveyed. Blood samples and peripheral leukocytes were analyzed for blood lead concentrations, telomere length, Comet assay, and cytokinesis-block micronucleus assay. RESULTS:Blood lead concentration of exposed workers (3.08±1.86) mg/mL was significantly higher than that (0.26±0.25) mg/mL of the controls (P<0.05). In addition, tail lengthsand micronuclei frequencies in leukocytes from the exposed workers were significantly higher than that of the controls (P<0.05). However, relative telomere lengths from leukocytes of lead exposed workers were shorter than that of the controls(P<0.05). Correlation analyses indicated that micronucleus frequencies in binucleated cells, rate of micronucleated binucleate cells and rate of nucleoplasmic bridges were positively correlated with work age (P<0.05), and the rank correlation coefficient (rs) were 0.447, 0.432, 0.351 respectively. Work age and blood lead concentration were negatively correlated with relative telomere length of leukocytes (P<0.05), and rs were -0.306, -0.394 respectively. CONCLUSION:Lead exposure induced DNA damage, micronuclei and shorter telomere length in peripheral leukocytes of exposed workers.

Key words: telomere length, genetic damage, lead exposure, comet assay, cytokinesis-block micronucleus assay

CLC Number:

R994.6
Q355

LU Yao, LIU Xinxia, XING Xiumei, ZHAO Zhiqiang, OU Xiaoyan, SU Xiaolin, SUN Yi, CHEN Jingli, SHEN Biling, JIANG Jun, HE Yun. DNA damage and telomere length changes in peripheral leukocytes from lead exposed workers[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 179-184.

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DNA damage and telomere length changes in peripheral leukocytes from lead exposed workers

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