miR-338基因簇与食管癌发病风险的病例对照研究

doi:10.3969/j.issn.1004-616x.2016.02.005

Abstract

Abstract: OBJECTIVE: To investigate the expression level of miR-338 cluster in esophageal carcinoma and identify the relationship between miR-338 cluster and esophageal carcinoma. METHODS: A total of 86 cases of patients with confirmed esophageal carcinoma were selected, real time RT-PCR were performed to detect the expression of hsa-miR-338-3p, hsa-miR-338-5p and hsa-miR-657. We used paired t-test to analyze the expression of miRNA in tumor and adjacent non-tumor tissues, Pearson correlation analysis to examine the correlation of the expression of each miRNA and logistic regression analysis to analyze the effect of abnormal expression of miRNAs on the risk of esophageal cancer. RESULTS: hsa-miR-338-3p and hsa-miR-338-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues (P<0.05), and no statical difference was found of the expression of hsa-miR-657 between the two tissues (P>0.05). The expression of hsa-miR-338-3p and hsa-miR-338-5p were significantly correlated (r=0.754, P<0.05), and low expression of hsa-miR-338-5p was the risk factor for esophageal carcinoma (OR=1.264, P<0.05). CONCLUSION: miR-338 cluster may play an important role in the formation process of esophageal carcinoma. In addition hsa-miR-338-5p may represent the miR-338 cluster to be a useful biomarker for diagnosis.

Key words: miR-338, cluster, esophageal carcinoma, biomarker

CLC Number:

R730.2

GAO Zhikui, LIU Ran, YIN Lihong, PU Yuepu. A case-control study on the risk of esophageal carcinoma and miR-338 cluster[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 107-110.

References

[1] Reinhart BJ,Slack FJ,Basson M,et al. The 21-nucleotide let-7 RNA regulates developmental timing in caenorhabditis elegans[J]. Nature,2000,403(6772):901-906.
[2] Chen HH,Huang WT,Yang LW,et al. The PTEN-AKT-mTOR/RICTOR pathway in nasal natural killer cell lymphoma is activated by miR-494-3p via PTEN but inhibited by miR-142-3p via RICTOR[J]. Am J Pathol,2015,185(5):1487-1499.
[3] Li L,Chen YY,Li SQ,et al. Expression of miR-148/152 family as potential biomarkers in non-small-cell lung cancer[J]. Med Sci Monit,2015,21:1155-1161.
[4] Yue J,Tigyi G. Conservation of miR-15a/16-1 and miR-15b/16-2 clusters[J]. Mamm Genome,2010,21(1/2):88-94.
[5] Yu J,Wang F,Yang GH,et al. Human microRNA clusters:genomic organization and expression profile in leukemia cell lines[J]. Biochem Biophys Res Commun,2006, 349(1):59-68.
[6] Li Y,Li W,Ying Z,et al. Metastatic heterogeneity of breast cancer cells is associated with expression of a heterogeneous TGFβ-activating miR424-503 gene cluster[J]. Cancer Res, 2014,74(21):6107-6118.
[7] Lai M,Xiao C. Functional interactions among members of the miR-17-92 cluster in lymphocyte development,differentiation and malignant transformation[J]. Int Immunopharmacol,2015, 28(2):854-858.
[8] Krysan K,Kusko R,Grogan T,et al. PGE2-driven Expression of c-Myc and OncomiR-17-92 contributes to apoptosis resistance in NSCLC[J]. Mol Cancer Res,2014, 12(5):765-774.
[9] Villadsen SB,Bramsen JB,Ostenfeld MS,et al. The miR-143/-145 cluster regulates plasminogen activator inhibitor-1 in bladder cancer[J]. Br J Cancer,2012,106(2):366-374.
[10] Zhang J,Sun Q,Zhang Z,et al. Loss of microRNA-143/145 disturbs cellular growth and apoptosis of human epithelial cancers by impairing the MDM2-p53 feedback loop[J]. Oncogene,2013,32(1):61-69.
[11] Yang M,Liu R,Sheng J,et al. Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma[J]. Oncol Rep,2013, 29(1):169-176.
[12] Xue Q,Sun K,Deng HJ,et al. MicroRNA-338-3p inhibits colorectal carcinoma cell invasion and migration by targeting smoothened[J]. Jpn J Clin Oncol,2014,44(1):13-21.
[13] Huang N,Wu Z,Lin L,et al. MiR-338-3p inhibits epithelial-mesenchymal transition in gastric cancer cells by targeting ZEB2 and MACC1/Met/Akt signaling[J]. Oncotarget, 2015,6(17):15222-15234
[14] Li X,Li Z,Yang G,et al. MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo[J]. Mol Med Rep,2015,12(3):3951-3957.
[15] Yong FL,Law CW,Wang CW. Potentiality of a triple microRNA classifier:miR-193a-3p,miR-23a and miR-338-5p for early detection of colorectal cancer[J]. BMC Cancer, 2013,13(2):280.
[16] Chen Y,Chen J,Liu Y,et al. Plasma miR-15b-5p,miR-338-5p,and miR-764 as biomarkers for hepatocellular carcinoma[J]. Med Sci Monit,2015,21:1864-1871.
[17] 马旭怡. miR-338-5p靶向作用于TRAF3参与肾移植术后抗体介导的免疫排斥[D]. 苏州:苏州大学,2014.
[18] Yan W,Chen M,Tao Z,et al. Identification of predictive biomarkers for early diagnosis of larynx carcinoma based on microRNA expression data[J]. Cancer Genet,2013,206(9/10):340-346.
[19] Lv K,Guo Y,Zhang Y,et al. Allele-specific targeting of hsa-miR-657 to human IGF2R creates a potential mechanism underlying the association of ACAA-insertion/deletion polymorphism with type 2 diabetes[J]. Biochem Biophys Res Commun, 2008,374(1):101-105.
[20] Zhang L,Yang L,Liu X,et al. MicroRNA-657 promotes tumorigenesis in hepatocellular carcinoma by targeting transducin-like enhancer protein 1 through nuclear factor kappa B pathways[J]. Hepatology,2013,57(5):1919-1930.

A case-control study on the risk of esophageal carcinoma and miR-338 cluster

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHANG Chen, YANG Liyan, CAI Hongqing, YUAN Qing, HAO Jiajie, WANG Mingrong. Expressions and clinical implications of SERPINE1 mRNA in esophageal squamous carcinoma tissues [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 245-249.
[2]	DU Qiang, YAO Yiyong, ZENG Gang. Expression and clinical significance of ASPM in lung adenocarcinoma according to the TCGA database [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 457-463.
[3]	XU Haiming, LIU Keliang, HAO Yanxing, WANG Ling, LIU Zhihong, DE Xiaoming. Usefulness of combined application of biomarkers in early auxiliary diagnosis of silicosis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 395-397,401.
[4]	ZHU Yiqing, ZHANG Tongtong, ZHENG Junwen, HAO Jiajie, CAI Yan, WANG Mingrong, LIANG Jianwei, ZHANG Yu. Expression status of p-mTOR and its prognostic implication in colorectal cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 161-165.
[5]	ZHAO Hua, LU Xue, CAI Tianjing, LI Shuang, TIAN Mei, LIU Qingjie. Screening for radiation-sensitive biomarkers in rat plasma based on metabolomics studies [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 166-170,176.
[6]	SUN Xiaonan, YUAN Qing, YANG Liyan, ZHANG Yu, CAI Yan, XU Xin, WANG Mingrong, HAO Jiajie, JIA Xuemei. FGFBP2 over-expresssion promoted cell proliferation in esophageal squamous carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 180-185,192.
[7]	ZHANG Haiyan, LI Qingye, CHEN Shen, CHEN Wen, HE Zhini. Construction of an environmental pollution-responsive gene-specific pyrosequencing assay [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 81-86.
[8]	ZHENG Jian, LAN Tao, LIU Wei, DENG Rongxia, LI Peimao, ZHANG Yanfang, LIU Jianjun. Differentially expressed miRNA profile in workers with trichloroethylene-induced medicamentosa-like dermatitis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 468-471.
[9]	Bernd Kaina. DNA repair in cancer therapy with methylating anticancer drugs: improving therapeutic efficacy and reducing side effects [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(5): 333-341,347.
[10]	CAI Xiaoman, CHEN Suzuan, YU Jing, LIU Zhaohui, WU Ruinuan, TANG Shengxin, GUO Guanghua. The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 227-230.
[11]	LI Lin, LI Xiaoxin, LIN Zhongwu, HU Jiangwei, ZHANG Xueqing, SHEN Liping, CHANG Cheng, CHEN Ying, GE Yinlin, WANG Zhidong. Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 421-425,431.
[12]	CHEN Haijun, LIU Yuqin, LI Yuze, WANG Xin, LI Nan, LI Dianzhong. Vitamin E succinate inhibits proliferation and induces apoptosis in human esophageal carcinoma cells TE-1 and Eca-109 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 357-360.
[13]	SHI Wen-rong，LIU Yan*. Effects of methyl benzyl nitrosamine on β-catenin and p-β-catenin(Ser675) in esophageal precancerous lesions in Wistar rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(5): 334-338.
[14]	LIU Hai-ying，HUANG Qing，DONG Jing-wen，LI Li*. Oct4 regulates the miR-302 promoter in F9 mouse embryonic carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(4): 258-265.
[15]	GUO Xia，JIN Hua，Mayila·KAMILIJIANG，LIU Kai-jiang，Axiangu·HASIMU,Halidan·REYIMU，WU Gui-zhen，Abulizi·ABUDULA. Correlation of the dynamic changes of plasma apoA l content and mTOR activity with cervical carcinogenesis development in Uyghur women [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(3): 185-192.