中国地鼠口腔颊囊黏膜癌模型的建立及癌变的动态观察

doi:10.3969/j.issn.1004-616x.2016.01.012

Abstract

Abstract: OBJECTIVE: To observe the dynamic histological changes in dimethylbenzanthracene(DMBA)-induced cancer in the Chinese hamster's buccal pouch mucosa cancer experimental animal model. METHODS: 60 male Chinese hamsters were randomly divided into three groups;experimental group(24 hamsters),solvent control group (12 hamsters) and control group(24 hamsters). In the solvent control group,the site was only coated with acetone solution. The buccal pouch mucosas were treated with 0.5% DMBA 3 times a week for 15 weeks. For the experimental and the control groups,6 hamsters were randomly selected and killed after 6 weeks,9 weeks,12 weeks and 15 weeks. For the solvent control group,3 hamsters were killed each time. The morphological and histological changes of buccal pouch mucosa were dynamic observed under naked eye and light microscope. RESULTS: Compared with the control group,a series of abnormal changes was consecutively observed in the experimental group;hyperemia,incrassation,festering Canker leukoplakia Cauliflower shape vegetations. The stages of histological changes had the simple hyperplasia(6^th-9^th week),the epithelial dysplasia of different degrees(9^th-12^th week),carcinoma in situ and squamous cell carcinomas (12^th-15^th week). CONCLUSION: Our experimental model shows similar changes to that of human oral squamous cell carcinoma. Therefore,the model can be used to study oral squamous cell carcinoma in multi-stage and multi-step dynamic development.

Key words: Chinese hamster, buccal pouch mucosa, oral squamous cell carcinoma, dimethylbenzanthracene, animal model

CLC Number:

R73-3

HUANGFU Bing, PANG Wenbiao, ZHANG Ruihu, GUO Min, GAO Jiping, SONG Guohua. Development of oral buccal pouch mucosa cancer in Chinese hamsters and dynamic observation of their carcinogenesis[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 56-59,65.

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Development of oral buccal pouch mucosa cancer in Chinese hamsters and dynamic observation of their carcinogenesis

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