阿特拉津对SD大鼠黑质TH和Nurr1基因表达的影响

doi:10.3969/j.issn.1004-616x.2016.01.005

Abstract

Abstract: OBJECTIVE: We evaluated the effects of atrazine(ATR) exposure in Sprague Dawley rat on the dopaminergic system. METHODS： Healthy adult male SD rats were randomly divided into four groups,control and ATR-dose (50,100,200 mg/kg) groups. Rats were treated orally with ATR for 28 d. The mRNA and protein expression of tyrosine hydroxylase (TH) and nuclear receptor-related factor 1 (Nurr1) were examined in samples of the substantia nigra by fluorescence PCR and immunohistochemistry. We use SPSS 17.0 to analyse the experimental results. RESULTS： qPCR results showed that with 50,100 and 200 mg/kg concentration of ATR exposure,rat substantia nigra TH and Nurr1 mRNA expression was significantly reduced compared with control group (P<0.05),and there was a dose-response relationship. Immunohistochemistry showed that substantia nigra Nurr1 and TH proteins in treated-rats were significantly lower than those among the control group (P<0.05). CONCLUSION： The reduction of Nurr1 and TH suggests that ATR may influence the metabolism of dopamine neurons in dopaminergic neuron injury.

Key words: atrazine, dopaminergic neurons, tyrosine hydroxylase, nuclear receptor-related factor 1

CLC Number:

R114

HE Xi, LI Yanshu, LI Baixiang. Effect of atrazine on expression of TH and Nurr1 genes in the substantia nigra of Sprague-Dawley rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 23-26,31.

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Effect of atrazine on expression of TH and Nurr1 genes in the substantia nigra of Sprague-Dawley rats

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