磷化铟/硫化锌量子点对小鼠腹腔巨噬细胞功能的影响

doi:10.3969/j.issn.1004-616x.2016.01.004

Abstract

Abstract: OBJECTIVE: To investigate the effects of InP/ZnS quantum dots (InP/ZnS QDs) on function of macrophages using in vitro cell model. METHODS: The mouse macrophage RAW264.7 cells were exposed to different concentrations of InP/ZnS QDs. The uptake of InP/ZnS QDs by macrophages was observed under a fluorescence microscope 4 hours later. Effect of InP/ZnS QDs on cell proliferation was assessed by CCK-8 assay,and the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) released by macrophages were determined by ELISA assay. RESULTS: InP/ZnS QDs entered the cells and remained mostly in the cytoplasm in 4 hours. Cells exposed to InP/ZnS QDs showed significantly decreased cell reproduction 24 hours and 48 hours later(P<0.05,P<0.01),and the levels of TNF-α released with or without CpG-ODN stimulationby macrophages significantly increased 24 hours later(P<0.01),but the levels of IL-6 remained unchanged. In addition,InP/ZnS QDs exposure led to increased release of TNF-α by macrophages following re-stimulation with CpG-ODN. CONCLUSION: The results suggest that InP/ZnS QDs could be taken up by macrophages,and could inhibit the cell viability and increase release of TNF-α.

Key words: InP/ZnS, quantum dots, macrophages, cytotoxicity, cytokine

CLC Number:

Q291

ZHENG Zhijia, TIAN Jinglin, MEI Shujiang, LIN Guimiao, WANG Xiaomei, ZHU Xuedan, TANG Jie, ZHANG Ke, CHEN Xianxiong. Effects of InP/ZnS quantum dots on function of mouse macrophage cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 19-22.

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Effects of InP/ZnS quantum dots on function of mouse macrophage cells

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