EGCG对顺铂损伤HEK293细胞及杀伤A549细胞的影响

doi:10.3969/j.issn.1004-616x.2016.01.003

Abstract

Abstract: OBJECTIVE: To investigate the effects of epigallocatechin gallate (EGCG) on cisplatin (DDP)-induced cytotoxicity in human embryo kidney (HEK) 293 cells and A549 cells. METHODS: HEK293 cells and A549 cells were cultured in vitro,and effects of DDP and EGCG on HEK293 cells and A549 cells were observed by MTT. The cells were divided into control group,DDP group and DDP+EGCG group. Then,the rates of HEK293 and A549 cell death were investigated by MTT. RESULTS: The IC₅₀ of EGCG on HEK293 cells was 61.6 mg/L. At lower than 40 mg/L,EGCG had no significantly effects on DDP-induced HEK239 cell death. At higher than 40 mg/L,EGCG significantly enhanced DDP-induced HEK239 cell death. The IC₅₀ of EGCG on A549 cells was 33.6 mg/L. At higher than 40 mg/L,EGCG significantly enhanced DDP-induced A549 cell death. CONCLUSION: EGCG had no significant protective effects on DDP-induced HEK239 cell death,but EGCG induced more cytotoxicity on cancer cells than on normal cells. When co-treated with DDP,A549 cell sustained more damage than HEK239 cells.

Key words: epigallocatechin gallate, cisplatin, nephrotoxicity, anticancer

CLC Number:

Q946.8

LIAN Yanna, GUO Shuqin, ZHOU Qiyun, GAO Zhaolan, WANG Hai, GAO Liping. Effects of epigallocatechin gallate on cisplatin-induced cytotoxicity in HEK293 and A549 cell lines[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 14-18.

References

[1] Abrams TJ,Lee LB,Murray LJ,et al. SU11248 inhibits KIT and platelet-derived growth factor receptor beta in preclinical models of human small cell lung cancer[J]. Mol Cancer Ther 2003,2(5):471-478.
[2] Maurmann L,Belkacemi L,Adams NR,et al. A novel cisplatin mediated apoptosis pathway is associated with acid sphingomyelinase and FAS proapoptotic protein activation in ovarian cancer[J]. Apoptosis,2015,20(7):960-974.
[3] Chau C,Wheater M,Geldart T,et al. Clinical outcomes following neoadjuvant cisplatin-based chemotherapy for bladder cancer in elderly compared with younger patients[J]. Eur J Cancer Care (Engl),2015,24(2):155-162.
[4] Prabhakaran P,Hassiotou F,Blancafort P,et al. Cisplatin induces differentiation of breast cancer cells[J]. Front Oncol 2013,3:134.
[5] Rodriguez-Fernandez E,Manzano JL,Alonso A,et al. Fluorescent cisplatin analogues and cytotoxic activity[J]. Curr Med Chem,2009,16(32):4314-4327.
[6] 熊立瑰,刘仲华,黄建安. 茶儿茶素研究进展[J]. 茶叶通讯,2011,38(1):27-31.
[7] Muller P,Downard KM. Catechin inhibition of influenza neuraminidase and its molecular basis with mass spectrometry [J]. J Pharm Biomed Anal,2015,111:222-230.
[8] Kobayashi H,Tanaka Y,Asagiri K,et al. The antioxidant effect of green tea catechin ameliorates experimental liver injury [J]. Phytomedicine,2010,17(3/4):197-202.
[9] Cueno ME,Tamura M,Ochiai K. Middle-aged rats orally supplemented with gel-encapsulated catechin favorably increases blood cytosolic NADPH levels[J]. Phytomedicine,2015 22(4):425-430.
[10] Suganuma M,Saha A,Fujiki H. New cancer treatment strategy using combination of green tea catechins and anticancer drugs[J]. Cancer Sci,2011,102(2):317-323.
[11] 赵艳萌,赵江燕,高丽萍. 葡萄籽原花青素对顺铂所致大鼠肾脏氧化损伤和线粒体损伤的防护作用[J]. 现代食品科技,2015,31(1):6-10.
[12] Santos NA,Catao CS,Martins NM,et al. Cisplatin-induced nephrotoxicity is associated with oxidative stress,redox state unbalance,impairment of energetic metabolism and apoptosis in rat kidney mitochondria[J]. Arch Toxicol,2007,81(7): 495-504.
[13] Hu J,Zhou D,Chen Y. Preparation and antioxidant activity of green tea extract enriched in epigallocatechin (EGC) and epigallocatechin gallate (EGCG)[J]. J Agric Food Chem,2009 57(4):1349-1353.
[14] 张媛媛,侯卫平,袁发. EGCG对肾脏疾病保护作用的研究进展[J]. 中国中西医结合肾病杂志,2013,14(11):1022-1024.
[15] Dasari S,Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action[J]. Eur J Pharmacol,2014 740:364-378.
[16] 凌兵,冯林,程书钧. DENND2D通过调控PARP1对非小细胞肺癌细胞系H1299中顺铂细胞毒性的影响[J]. 癌变·畸变·突变,2013,25(2):87-90.
[17] 张焕金,蔡林儿. 绿茶提取物茶多酚抗癌作用的研究进展[J]. 华南国防医学杂志,2012,26(5):522-523.
[18] 吴方红,周学军,文静. EGCG对大肠癌细胞系LoVo细胞增殖的影响及其机制[J]. 辽宁中医药大学学报,2015,17(1): 33-36.
[19] Zhang Y,Wang X,Han L,et al. Green tea polyphenol EGCG reverse cisplatin resistance of A549/DDP cell line through candidate genes demethylation[J]. Biomed Pharmacother 2015,69: 285-290.

Effects of epigallocatechin gallate on cisplatin-induced cytotoxicity in HEK293 and A549 cell lines

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yuqiang, LI Hongjuan, JIANG Tianliang, CAO Yifei, YANG Jun. Involvement of UBE2A in cisplatin-induced stress response in HeLa cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 463-467.
[2]	HAN Hedan, WANG Hai, LI Ailin, HAN Zhaoyu, GAO Liping. Protective effect of Rhodiola extract on cisplatin-induced nephrotoxicity [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 204-208.
[3]	HAN Hedan, WANG Hai, GAO Liping. Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 20-24.
[4]	ZHOU Qingbiao, LIU Ying, KONG Deqin, ZHANG Jiaxin, YU Weihua, BAI Hua, HAI Chunxu. Protective effect of selenium in chronic cadmium exposure-induced kidney injury in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 1-7.
[5]	WANG Hai, LIAN Yanna, GAO Liping, QU Lijie, ZHANG Dongping. Effect of catechin on cisplatin-induced oxidative damage in human embryonic kidney cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 51-54.
[6]	YANG Meiling, LI Xin, HUANG Junming, ZHANG Zihong, YI Zimei, GU Meiying, HUANG Jiankang, HE Yun. Enhancement of anti-tumor effect of cisplatin by chrysin in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 37-41,59.
[7]	WU Huatao, SHEN Jiaxin, LIU Jing. Overexpression of Notch3 enhance the efficacy of cisplatin to MDA-MB-231 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 111-114,120.
[8]	TENG Qian, MA Tingting, SUN Yongye, ZHANG Huaqi, MA Aiguo. Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 370-374.
[9]	YAN Jin, ZHAO Xiaoting, JIANG Mei, GU Meng, YUE Wentao. High temperature, ultraviolet light and cisplatin caused apoptosis of H1299 cell line which over expressed cyclophilin A [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 177-181,186.
[10]	NI Juan，LI Zhen，XUE Jing-lun，WANG Xu. Effects of (-)epigallocatechin gallate on expressions of hMLH1 and hMSH2 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(4): 276-279.
[11]	LING Bing，FENG Lin，CHENG Shu-jun. Effect of DENND2Don the cisplatinum cytotoxicity in H1299 cells by regulating PARP1 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(2): 87-90.
[12]	HUANG Da-wei，CHEN Yang-ming，ZHANG Xin，GONG Zhi-yong. The combined acute oral toxicity of melamine and cyanuric acid in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 61-63.
[13]	YU Li;LING Yun-zhi. Effect of cisplatin on the expression of SATB1 in SMMC-7721 cells [J]. , 2012, 24(4): 275-278.
[14]	LI Zhan-jun，LIN Fei，FAN Hui-hong，XU Kang-sen. The anticancer effect of serum thymic factor 9 peptide on human colon cancer HT-29 [J]. , 2011, 23(5): 374-376.
[15]	JIN Yi-feng1, LI Tie-chen2, WANG Ying1,,LIU Dong-hua11,SUN Zhen-gui1,. Single nucleotide polymorphisms in ERCC1 and XPD genes and sensitivity to platinum_based chemotherapy in non_small_cell lung cancer [J]. , 2010, 22(5): 374-378.