GPX1过表达对肺癌BERP35T1细胞DNA氧化损伤和恶性表型的影响

doi:10.3969/j.issn.1004-616x.2016.01.002

Abstract

Abstract: OBJECTIVE: To study the effects of glutathione peroxidase 1(GPX1) overexpression on DNA oxidative damage level and phenotype of malignant BERP35T1 cells exposed upon α-particles. METHODS: The eukaryotic expression vector pEGFP-GPX1 was constructed. After PCR,enzyme digestion analysis and sequencing pEGFP-GPX1 and control vector were transfected into BERP35T1 cells with lipofectamine 2000 and G418 screening was used to obtain resistant cell lines. The expression of GPX1 was measured by Western blot. MTT,scratching healing test and anchorage independence growth test were used to analyze the effects of GPX1 overexpression on growth rate,migration and colony forming efficiency of BERP35T1 cells. RESULTS: pEGFP-GPX1 was confirmed by PCR,enzyme digestion analysis and sequencing. The sequence of the target gene GPX1 was entirely correct. The protein expression level of GPX1 in pEGFP-GPX1 transfected group BERP35T1-GPX1-6 was 4.01 times higher than in BERP35T1-pEGFP cells(P<0.05). Compared with BERP35T1 and BERP35T1-pEGFP,the level of growth rate,migration and colony forming efficiency in BERP35T1-GPX1-6 decreased significantly(P<0.05). CONCLUSION: GPX1 overexpression may inhibit the proliferation and metastasis of malignant BERP35T1 cells exposed to α-particles through reducing the level of DNA oxidative damage.

Key words: glutathione peroxidase 1, α-particle, lung cancer, oxidative stress

CLC Number:

R994.6

SHAO Shuai, WEI Zhiquan, ZHANG Wei, TONG Peng, WANG Chunyan, QI Xuesong, GOU Qiao. Effects of glutathione peroxidase 1 overexpression on DNA oxidative damage and phenotype of malignant BERP35T1 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(1): 8-13,18.

References

[1] Hajo Z,Ferid S. WHO Handbook on Indoor Radon[M]. Paris:WHO Press,2009:3-14.
[2] 叶常青,柳伟伟,胡良平,等. 对氡致肺癌病因判断若干问题的探讨[J]. 中华放射医学与辐射防护杂志,2012,32(2): 318-322.
[3] 陈志海,聂继华,武彦文,等. 氡吸入染毒致大鼠相关靶器官的损伤[J]. 癌变·畸变·突变,2011,23(6):460-464.
[4] Sun JF,Sui JL,Zhou PK,et al. Decreased efficiency of gamma-ray-induced DNA double-strand break rejoining in malignant transformants of human bronchial epithelial cells generated by alpha-particle exposure[J]. Int J Radiat Biol 2002,78(9):773-780.
[5] 苟巧,张伟,王春燕,等. α粒子诱发BEP2D细胞恶性转化中抗氧化能力和辐射敏感性研究[J]. 中华放射医学与防护杂志,2011,31(1):1-5.
[6] 苟巧,佟鹏,张翠兰,等. α粒子诱发BEP2D细胞恶性转化中细胞内抗氧化蛋白和活性氧水平研究[J]. 癌变·畸变·突变,2011,23(6):411-415.
[7] 苟巧,王春燕,张翠兰,等. α粒子诱发BEP2D细胞恶性转化中脂质和DNA氧化损伤的研究[J]. 中华放射医学与防护杂志,2012,32(2):157-161.
[8] Thu VT,Kim HK,Ha SH,et al. Glutathione peroxidase 1 protects mitochondria against hypoxia/reoxygenation damage in mouse hearts[J]. Pflugers Arch,2010,460(1):55-68.
[9] Nalkiran I,Turan S,Arikan S,et al. Determination of gene expression and serum levels of MnSOD and GPX1 in colorectal cancer[J]. Anticancer Res,2015,35 (1):255-259.
[10] Lou T,Xiang X,Wu D. Transformation of human bronchial epithelial cells BEP2D induced by 238Pu α-particles[J]. Chin J Lung Canc,2000,3(6):428-431.
[11] Kumar A,Pant MC,Singh HS,et al. Determinants of oxidative stress and DNA damage (8-OhdG) in squamous cell carcinoma of head and neck[J]. Indian J Cancer,2012 49(3):309-315.
[12] Xu X,Wang Y,Guo W,et al. The significance of the alteration of 8-OHdG in serous ovarian carcinoma[J]. J Ovarian Res,2013,6(1):74.
[13] 周平坤. α粒子辐射致癌的遗传不稳定性机制研究[D]. 北京:中国人民解放军军事医学科学院,2004.

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Effects of glutathione peroxidase 1 overexpression on DNA oxidative damage and phenotype of malignant BERP35T1 cells

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