某氡温泉周边居民外周血淋巴细胞中miRNAs的表达

doi:10.3969/j.issn.1004-616x.2015.06.008

Abstract

Abstract: OBJECTIVE: To investigate the expressions of miR-16,miR-106b,miR-449a,miR-34a and let-7g in peripheral blood lymphocytes of the residents around hot springs with radon in Hebei province. METHODS: Two groups were randomly selected in Hebei province,Radon group consisted of 43 residents around hot springs with radon and control group were 44 residents with similar living conditions but not around hot springs. qRT-PCR was used to detect miRNAs level in radon and control groups. t test was used for statistical analysis of miRNA levels in lymphocytes between two groups. Multiple linear regression analyse were employed to test the association between miRNAs expression and radon exposure. RESULTS: Compared with control group,the levels of miR-106b,miR-449a and let-7g in lymphocytes of radon group were significantly increased(t=4.180,2.422,2.794;P<0.05). After controlling of confounding factors such as age,gender,smoking and drinking,alteration of miR-106b,miR-449a and let-7g in lymphocytes were positively correlated with radon exposure(t=4.091,2.716,2.440; P<0.05). No significant difference was observed in the lymphocyte levels of miR-16 and miR-34a between the two groups(t=0.990,0.147;P>0.05). CONCLUSION: MiR-106b,miR-449a and let-7g levels could potentially used as biomarkers for radon exposure.

Key words: hot springs with radon, lymphocyte, miRNAs, radiation injury

CLC Number:

Q345+.1

LIU Chunxu, LI Xiaoliang, PAN Yan, RUAN Jianlei, GAO Gang, TIAN Mei, LIU Jianxiang. Expression of miRNAs in peripheral blood lymphocytes of the residents around hot springs with radon[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 446-449.

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Expression of miRNAs in peripheral blood lymphocytes of the residents around hot springs with radon

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