VEGF介导微囊藻毒素-LR促进结直肠癌细胞DLD-1侵袭迁移的研究

doi:10.3969/j.issn.1004-616x.2015.06.007

Abstract

Abstract: OBJECTIVE: This study investigated whether microcystin-LR regulated the expression of VEGF and promoted invasion and migration in colorectal DLD-1 cells. METHODS: Transwell experiments were used to analyze the invasion and migration in colorectal cancer DLD-1 cells treated with different concentrations of microcystin-LR. Expression of VEGF in treated cells was evaluated by real-time quantitative PCR(qPCR),Western blot and ELISA. RESULTS: That the abilities of invasion and migration improved significantly in microcystin-LR-treated groups,compared wiose in the control group. When the concentration of microcystin-LR reached 12.5 nmol/L,invasion and migration were increased to 2.1 and 2.0 folds,respectively. The qPCR and Western blot results suggested that microcystin-LR upregulated the expression level of VEGF mRNA and protein in DLD-1 cells. When microcystin-LR concentration reached 12.5 and 25 nmol/L,the expression levels of the VEGF protein and mRNA in DLD-1 cells reached a maximum of 1.7 and 2.8 folds increases,respectively. Mean while,the significant 1.5-foldelevated VEGF production in the culture medium was also detected. CONCLUSION: Microcystin-LR upregulation of VEGF was involved in promoting invasion and migration in colorectal cancer cells DLD-1.

Key words: microcystin-LR, colorectal cancer, invasion and migration, vascular endothelial growth factor

CLC Number:

R73-37

REN Yan, QIN Wei, ZHU Qiangqiang, ZHOU Lihua, WANG Ting. VEGF expression in microcystin-LR promotion of invasion and migration in DLD-1 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 441-445.

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VEGF expression in microcystin-LR promotion of invasion and migration in DLD-1 cells

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