肺癌患者MPDZ基因异常甲基化的研究

doi:10.3969/j.issn.1004-616x.2015.06.006

Abstract

Abstract: OBJECTIVE: To study MPDZ gene hypermethylation in lung cancer tissue,peripheral plasma and sputum,and to explore its relationship with clinicopathologic features. METHODS: Methylation specific PCR was used to detect the methylation of MPDZ gene in 49 cases of lung cancer tissue,20 cases of normal tissue and corresponding blood plasma and sputum samples. RESULTS: The frequency of methylation of MPDZ gene promoter was 67%(33/49) in lung cancer tissues,but not in normal tissues 0(0/20)(P=0.00). The methylation of MPDZ gene was significantly correlated with clinic stages of lung cancer patients(P=0.039),but not correlated with patient's age,sex,smoking,tumor differentiation and pathological classification(P>0.05). In 33 patients with MPDZ hypermethylation in tumor tissue,the same aberrant methylation was detected in 25(76%) of the corresponding plasma and 18(55%) of sputum samples,whereas hypermethylation of MPDZ was not found in the sputum and plasma from the patients with MPDZ unmethylation in tumor sample. CONCLUSION: MPDZ gene has highly methylated in lung cancer tissue,corresponding blood plasma and sputum. The methylation of MPDZ gene may have a certain value in the diagnosis of lung cancer.

Key words: MPDZ gene, lung cancer, DNA methylation, methylation specific PCR

CLC Number:

R730.2

FENG Jie, LIU Wenbin, CHEN Hongqiang, HUANG Yongsheng, HAN Fei, CAO Jia, LIU Jinyi. DNA hypermethylation status of MPDZ gene in lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 437-440.

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DNA hypermethylation status of MPDZ gene in lung cancer

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