甲基丙烯酸环氧丙酯致16HBE细胞恶性转化不同时期METTL9基因甲基化状态分析

doi:10.3969/j.issn.1004-616x.2015.06.005

Abstract

Abstract: OBJECTIVE: To analyze the methyltransferase like 9(METTL9) methylation status at different stages of malignant transformation of human bronchial epithelial cells(16HBE) induced byglycidyl methacrylate(GMA) and to explore the effect of METTL9 methylation in the process of malignant transformation. METHODS: Cells were harvested th in the 10^th generation(early stage),the 20^th generation(mid stage) and the 30^th generation(later period). To verify the methylation chip result of METTL9 methylation status in the process of malignant transformation,real-time fluorescence quantitative PCR(qPCR) was applied to measure the gene expression levels of METTL9 at different transformed stages,and compared wie control groups(treated with DMSO). RESULTS: Based on the result of methylation chip,methylation of METTL9 gene occurred in the 10^th and 20^th generations(PeakScore>2). qPCR showed that the levels of gene expression increased in the 10^th and 20^th generation in GMA group(P<0.05). The level of METTL9 gene expression increased in the 10^th generation(P<0.05),while decreased in the 20^th generation after treatment with 5-Aza-cdr(P<0.05). CONCLUSION: Methylation status of METTL9 gene promoter could be considered as a stable and specific biomarker in the lately and mid stages of transformation process.

Key words: glycidyl methacrylate, human bronchial epithelial cells, METTL9 gene, methylation

CLC Number:

R730.2

WANG Quankai, XIE Guangyun, LIU Hongmei, XU Jianning. METTL9 methylation status in malignant transformation of 16HBE cells induced by glycidyl methacrylate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 432-436.

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METTL9 methylation status in malignant transformation of 16HBE cells induced by glycidyl methacrylate

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