玉麦须醇提物对L929细胞氧化应激损伤的保护作用

doi:10.3969/j.issn.1004-616x.2015.06.004

Abstract

Abstract: OBJECTIVE: To investigate the protective effects of maize silks ethanol extract(MSE) against radiation-induced oxidative damage in cells. METHODS: γ-radiation was employed to induce oxidative stress in L929 cells. After radiation,cells were treated immediately with different doses of MSE(10,50,250μg/mL). The variation of oxidative damage indicator,antioxidant system and inflammatory factors were examined. RESULTS: Radiation caused obvious oxidative injury in cells. MSE treatment significantly abolished elevation of thiobarbituric acid reactive substances(TBARS) levels,increased hepatic GSH/GSSG ratio and activities of catalase and superoxide dismutase. Moreover,MSE up-regulated the gene expressions of NF-E2-related factor 2 and gamma glutamyl cysteine synthetase. The gene and protein expressions of transforming growth factor beta(TGF-β)were also inhibited. Interestingly,50μg/mL MSE treatment performed best in our most results. CONCLUSION: These findings proved the protective role of MSE against radiationinduced oxidative injury. Nrf2 and TGF-β could be the potential targets of maize silks.

Key words: maize silks, ⁶⁰Co radiation, oxidative stress, antioxidant

CLC Number:

Q345+.7

FANG Congwen, BAI Hua, ZHANG Xiaodi, LIU Rui, HAI Chunxu. Protective effects of maize silks ethanol extract on oxidative injury in L929 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 426-431.

References

[1] El-Ghorab A,El-Massry KF,Shibamoto T. Chemical composition of the volatile extract and antioxidant activities of the volatile and nonvolatile extracts of Egyptian corn silk(Zea mays L.)[J]. J Agric Food Chem,2007,55(22):9124-9127.
[2] Lin M,Chu QC,Tian XH,et al. Determination of active ingredients in corn silk,leaf,and kernel by capillary electrophoresis with electrochemicaI detection[J]. J Capill Electrophor Microchip Technol,2007,10:51-56.
[3] Velazquez DV,Xavier HS,Batista JE,et al. Zea mays L. extracts modify glomerular function and potassium urinary excretion in conscious rats[J]. Phytomedicine,2005,12(5):363-369.
[4] Maksimovic Z,Dobric S,Kovacevic N,et al. Diuretic activity of Maydis stigma extract in rats[J]. Pharmazie,2004,59:967-971.
[5] 刘娟,韩晓强,姜博.玉米须多糖治疗糖尿病作用研究[J].时珍国医国药,2006,17(8):1441-1442.
[6] 岳淑玲,兰翠,李金梅.玉米须治疗慢性胆道感染[J].中国民间疗法,2007,15(11):30-31.
[7] 柏桦,海春旭,梁欣,等.玉麦须提取物清除自由基和抑制脂质过氧化作用[J].癌变·畸变·突变,2008,19(1):147-151.
[8] Bai H,Hai C,Xi M,et al. Protective effect of maize silks(Maydis stigma) ethanol extract on radiation-induced oxidative stress in mice[J]. Plant Foods Hum Nutr,2010,65(3):271-276.
[9] 海春旭,常耀明,王枫.高原缺氧/再给氧毒性损伤与脂质过氧化作用的关系[J].卫生毒理学杂志,2001,15(3):60-61.
[10] 秦绪军,何伟,海春旭,等.复合抗氧化剂对荷瘤大鼠⁶⁰Co γ射线局部照射时血液氧化损伤的影响[J].第四军医大学学报,2002,23(9):850-852.
[11] 庞战军,周玫,陈瑗.自由基医学研究方法[M].北京:人民卫生出版社,2000:268-271.
[12] Danyelle M,Tew KD,Tapiero H,et al. The importance of glutathione in human disease[J]. Biomed Pharmacothera, 2003,57(3/4):145-155.
[13] Seo YJ,Lee JW,Lee EH,et al. Role of glutathione in the adaptive tolerance to H₂O₂[J]. Free Radical Biol Med,2004, 37(8):1272-1281.
[14] Kensler TW,Wakabayashi N,Biswal S. Cell survival responses to environmental stresses via the Keap1-Nrf2-ARE pathway[J]. Annu Rev Pharmacol Toxicol,2007,47:89-116.
[15] Scibior D,Czeczot H. Catalase:structure,properties, functions[J]. Postepy Hig Med Dosw,2006,60:170-180.
[16] Teshima K,Yamamoto A,Yamaoka K,et al. Involvement of calcium ion in elevation of mRNA for gammaglutamylcysteinesynthetase(gamma-GCS) induced by low-dose gamma-rays[J]. Int J RadiatBiol,2000,76(12):1631-1639.
[17] Li MO,Flavell RA. Contextual regulation of inflammation:a duet by transforming growth factor-beta and interleukin-10[J]. Immunity,2008,28(4):468-476.

Protective effects of maize silks ethanol extract on oxidative injury in L929 cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	AI Duo, XU Anqi, KONG Deqin, LIU Ying, YU Weihua, WANG Zhao, PENG Jie, LIU Rui, ZHANG Xiaodi, HAI Chunxu, LI Wenli, WANG Xin, LIU Jiangzheng. Protective effects of catalytic antioxidant AEOL-10150 against nitrogen mustard-induced acute liver injury in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 40-46,61.
[2]	LI Hongwei, KONG Deqin, YU Weihua, WU Hao, WANG Zhao, LIU Rui, HAI Chunxu, WANG Xin, LIU Jiangzheng, LI Wenli. Protective effect of oleanolic acid co-treatment on alcohol-induced oxidative damage in gastric mucosa of rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 365-369.
[3]	JIANG Shan, DU Feng, KANG Bingwen, YIN Caocao, ZHOU Jian, SHI Ying, WANG Yue, ZHOU Gengyao, QIN Xujun. Attenuation of D-galactose-induced senescence of human umbilical vein endothelial cells by isorhamnetin [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 95-100.
[4]	ZHAO Junwei, HUA Chenfeng, SHANG Pingping, XIE Fuwei, LI Xiang. Induction of oxidative stress by arsenious acid As(Ⅲ) in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 28-31.
[5]	SUN Jiaojiao, CHE Bizhong, LUO Qiulin, ZHAI Bingzhong, XIN Lili. 1,25-(OH)₂D₃ attenuates PM_2.5-induced oxidative stress in HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 92-97.
[6]	CHEN Zizhuo, XU Yuhang, ZHAO Jiuzhou, ZHU Jingpu, ZHENG Yipeng, LI Wenli, WU Haozhi, HAI Chunxu, YU Weihua. Antioxidant and anti-inflammatory effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in macrophages [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(2): 105-111,117.
[7]	JIN Lei, ZHOU Jiayou, HAN Jiacheng, GUAN Haojun, WANG Shuai, PENG Jie, WANG Xin, HAI Chunxu, LI Wenli. Protective effects of Jiangzhitong on palmitic acid-induced insulin resistance in BRL-3A cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 21-28.
[8]	ZHANG Xuguang, DU Ruihu, CHEN Yanping, ZHAO Yan. Effect from activation of acid sphingomyelinase and ceramide on vitamin E succinate-induced apoptosis in human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 379-384.
[9]	LIAO Nai, LONG Zi, HAI Chunxu, WANG Xin. 2,3,7,8-tetrachlorodibenzo-p-dioxin alone or in combination with high fat diet on glucose and lipid metabolism in mice [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(2): 111-118.
[10]	GUO Haidong, YANG Runfang, LIU Xinyu, LIU Junjun, HE Jin, GAN Ziqiong, LI Hongxia. Oxidative stress response in rats after exposure to fire-drill prop smoke [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 41-44,48.
[11]	WEN Hairuo, GUO Yajuan, HUANG Zhiying, WANG Xue, DAN Mo. Induction of cytotoxicity and DNA damage by iron oxide nanoparticles with different surface modifications in A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 413-421.
[12]	ZHOU Xiaoqing, WEN Kexin, YIN Hongping, YANG Jinru, ZHU Yongfei. Effects of intrauterine flutamide exposure on uterus and ovary development and on oxidative stress among female offspring [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 384-388,394.
[13]	HAN Hedan, WANG Hai, LI Ailin, HAN Zhaoyu, GAO Liping. Protective effect of Rhodiola extract on cisplatin-induced nephrotoxicity [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(3): 204-208.
[14]	WANG Yuan, DENG Xiaofei. Effect of DNA glycosylase OGG1 against Helicobacter pylori-induced DNA damage in gastric epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 92-97,102.
[15]	HAN Hedan, WANG Hai, GAO Liping. Protective effects of allicin on cisplatin-induced toxicity in HEK293 kidney cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 20-24.