POLH mRNA作为辐射损伤分子生物标志物的可行性研究

doi:10.3969/j.issn.1004-616x.2015.06.003

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2015, Vol. 27 ›› Issue (6): 421-425,431.doi: 10.3969/j.issn.1004-616x.2015.06.003

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Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury

LI Lin¹, LI Xiaoxin², LIN Zhongwu³, HU Jiangwei⁴, ZHANG Xueqing², SHEN Liping², CHANG Cheng², CHEN Ying², GE Yinlin¹, WANG Zhidong²

1. Department of Biochemistry and Molecular Biology, College of Medical, Qingdao University, Qingdao 266021, Shandong;
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;
3. Ministry of Science and Technology, Academy of Military Medical Sciences, Beijing 100850;
4. 307 Affiliated Hospital of Military Medical Sciences, Beijing 100071, China

Received:2015-06-16 Revised:2015-10-27 Online:2015-11-30 Published:2015-11-30

Abstract

Abstract: OBJECTIVE: To explore the change in expression of POLH mRNA in human peripheral blood exposed to γ-ray and provide experimental evidence for POLH mRNA to be a new molecular biomarker for radiation injury. METHODS: After radiation with dose of 0,0.5,1,2,4,6,10 Gy by ⁶⁰Co γ-rays,total RNA was extracted from peripheral blood of three healthy adults at 0,2,4,8,12,24 h post-radiation. Expression level of POLH mRNA was measured by real-time quantitative PCR. 30 healthy donors' peripheral blood were collected as the background group to measure the expression level of POLH mRNA in different individuals. RESULTS: The expression level of POLH mRNA increased in a dose-dependent manner within the range of 0-2 Gy at 4,8,12,and 24 h after radiation,with no significant change at 2 h post-radiation. Over 2 Gy,the expression level of POLH mRNA was close to saturation. It also showed that the expression level of POLH mRNA increased in a time-dependent manner within the range of 4-12 h postradiation,a maximum response was observed at 12 h. The basal level of POLH mRNA was not affected by age and gender. CONCLUSION: POLH mRNA was induced by radiation and increased in dose-dependent and time-dependent manners. The back ground level in healthy human peripheral blood was uniform. Our results suggested that POLH mRNA may be a useful radiation biomarker.

Key words: radiation injury, molecular biomarker, POLH, real-time quantitative PCR

CLC Number:

Q345

LI Lin, LI Xiaoxin, LIN Zhongwu, HU Jiangwei, ZHANG Xueqing, SHEN Liping, CHANG Cheng, CHEN Ying, GE Yinlin, WANG Zhidong. Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(6): 421-425,431.

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Feasibility study on expression of POLH mRNA as a molecular biomarker for radiation injury

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