Carcinogenesis, Teratogenesis & Mutagenesis ›› 2015, Vol. 27 ›› Issue (5): 383-385,393.doi: 10.3969/j.issn.1004-616x.2015.05.012

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Impacts of leukocyte elevation on reactive oxygen species and sperm DNA damage

ZHANG Qiaoxin1, XIAO Yingxiu2, CHENG Bizhen1   

  1. 1. Department of Clinical Laboratory, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041;
    2. Department of Neurology, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2015-04-24 Revised:2015-09-10 Online:2015-09-30 Published:2015-09-30

Abstract:

OBJECTIVE: To study the impacts of leukocyte elevation on reactive oxygen species (ROS) and sperm DNA damage. METHODS:A total of 52 elevated leukocyte specimen and 50 normal leukocytes specimen were collected. ROS production was measured using luminol as the probe by the Chemiluminescence method. Sperm nuclear DNA damage was assessed by sperm chromatin structure assay (SCSA) and single-cell gel electrophoresis (SCGE). RESULTS:ROS levels (using the log-transformation) was significantly higher in the elevated leukocyte group (P<0.01). Data of SCSA indicated there were significant increases of DNA fragmentation index and high DNA stainable in the elevated leukocytes group (P=0.020, P=0.012). SCGE analysis, in terms of tail moments, was significantly higher in the elevated leukocyte group (P=0.007). CONCLUSION:Leukocyte elevation could result in the increase of reactive oxygen species, which may induce sperm nuclear DNA damage.

Key words: leukocytes, sperm, reactive oxygen species, DNA damage

CLC Number: