微囊藻毒素-LR所致神经小胶质细胞BV-2的炎性反应

doi:10.3969/j.issn.1004-616x.2015.05.010

Abstract

Abstract:

OBJECTIVE: To investigate the effects of mycrocystin-LR(MC-LR) on the neurotoxic mechanisms of the central nervous system, the contents of nitric oxide (NO), nitric oxide synthase (NOS), tumor necrosis factor-α (TNF-α), and interleukin- 6 (IL-6) in BV-2 microglial cells were detected in our study. METHODS:With the methyl thiazolyl tetrazolium (MTT) assay, the cytotoxicity of the inflammatory model in BV-2 cells was performed after exposure to MC-LR. The levels of NO and NOS were measured by the nitric acid reductase assay and nitric oxide synthase assay, respectively. The contents of TNF-α and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS:After exposure to MC-LR for 24 h, the viability in BV-2 cells was decreased with the increase of MC-LR concentration within the range of 0-28 μg/L (r=-0.609, P<0.05), and the median effective concentration (EC50) was 22.49 μg/L. Besides, the levels of IL-6 and NOS in BV-2 cells were increased at the concentration of 5-10 μg/L MC-LR, and the levels of TNF-α and NO were increased at the concentration of 10 μg/L MC-LR. Compared with control group, those differences above mentioned were statistically significant (P<0.05). CONCLUSION:MC-LR induced inflammatory response in BV-2 microglial cells by increases in production of NO, NOS, IL-6 and TNF-α.

Key words: microcystin-LR, BV-2 cells, neuroinflammation, nitric oxide, nitric oxide synthase

CLC Number:

R994.6

ZHANG Minhui, ZHOU Jue, LIU Ran, LI Xiaobo, LIANG Geyu, LI Yunhui. Effect of mycrocystin-LR on inflammatory response in BV-2 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 375-377,382.

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Effect of mycrocystin-LR on inflammatory response in BV-2 cells

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