补充叶黄素及EGCG对人体抗氧化活性及DNA氧化损伤的影响

doi:10.3969/j.issn.1004-616x.2015.05.009

Abstract

Abstract:

OBJECTIVE: To investigate the effects of lutein and epigallocatechin gallate (EGCG) supplementation on the antioxidant activity and DNA damage. METHODS:A total of 40 healthy young adults were randomly divided into four groups (n=10 in each group), including lutein group (20 mg/d), EGCG group (270 mg/d), lutein (20 mg/d)+EGCG (270 mg/d) group and normal control group. Three experimental groups of volunteers were treated with lutein and or EGCG for 20 d while the control group wasn't given any supplement. 5 mL fasting blood was collected at the beginning and the end of the trial. Plasma lutein and EGCG concentrations were measured by HPLC detector systems. Plasma total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondial dehyde (MDA) levels were measured by kits. Peripheral blood lymphocyte DNA damage was analyzed by single-cell gel electrophoresis (SCGE). RESULTS:After supplementation for 20 days, plasma lutein concentration of the subjects in the lutein group and lutein+EGCG group increased by 257% and 175%(P<0.01). Plasma MDA and T-SOD concentrations of the subjects in the lutein group were significantly lower than those of the control group, while plasma GSH-Px concentration was significantly increased (P<0.05). No significant difference was found in the antioxidant activity of the subjects in the EGCG group. The combined supplementation of lutein and EGCG had no interaction on the antioxidant activity. DNA damage analysis showed that low- and medium- concentration H₂O₂-induced DNA damage were markedly lower in either lutein or EGCG group than those in the control group (P<0.05). However high- concentration H₂O₂-induced and spontaneous DNA damage in both groups did not show any significant difference with those in the control group. The combined supplementation of lutein and EGCG had no interaction on the DNA damage (P>0.05). CONCLUSION:Lutein or EGCG supplementation alone could effectively reduce DNA damage. Lutein supplementation enhanced antioxidant activity, but EGCG did not. Lutein and EGCG did not show any interaction on antioxidant activity and DNA damage.

Key words: lutein, epigallocatechin gallate, antioxidation, DNA damage

CLC Number:

R151.3

TENG Qian, MA Tingting, SUN Yongye, ZHANG Huaqi, MA Aiguo. Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 370-374.

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Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage

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