TMEM196基因在肺癌中的甲基化修饰与表达调控

doi:10.3969/j.issn.1004-616x.2015.05.005

Abstract

Abstract:

OBJECTIVE: To analyze TMEM196 gene methylation and expression in lung cancer. METHODS: Methylation-specific PCR was used to detect the DNA methylation status of TMEM196 gene in normal and tumor tissues and cell lines. RT-PCR was used to analyze the expression of TMEM196 gene in tumor cells and also in 5-aza-dC-treated tumor cells. RESULTS:The hypermethylation of TMEM196 was detected in lung cancer tissues (57%, 28/49), but not in normal tissues (0, 0/20). Methylation of TMEM196 gene was significantly correlated with tumor differentiation (P=0.012) and the clinical stages of lung cancer patients (P=0.007), but not correlated with the patient's age, sex, smoking, and pathological classification (P>0.05). TMEM196 showed hypermethylation and its expression decreased in lung cancer cell lines H1975 and H1650. After treatment with demethylation drug, expression of TMEM196 gene increased significantly in these cell lines. CONCLUSION:The expression of TMEM196 gene was inhibited through hypermethylation in lung cancer. This process may play an important role in lung cancer.

Key words: TMEM196 gene, lung cancer, DNA methylation, expression

CLC Number:

R734.2

ZHAO Huan, LIU Wenbin, CHEN Hongqiang, HAN Fei, CAO Jia, LIU Jinyi. TMEM196 gene methylation and expression in lung cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 353-356.

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TMEM196 gene methylation and expression in lung cancer

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