Toll样受体2基因多态性与胃癌及EB病毒相关胃癌易感性的关系

doi:10.3969/j.issn.1004-616x.2015.05.003

Abstract

Abstract:

OBJECTIVE: The aim of our study was to evaluate the potential associations between the single nucleotide polymorphisms (SNPs) of Toll-like receptor (TLR2) rs3804099 and rs3804100 genes and the risk of gastric carcinoma(GC), especially to Epstein-Barr virus-associated gastirc carcinoma (EBVaGC). METHODS:TLR2 rs3804099 and rs3804100 gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 185 cases of EBV-negative GC, 41 cases of EBVaGC. 100 cases of peripheral blood samples from healthy individuals were also examined. The data was analysed by SPSS. RESULTS:As for the TLR2 gene (rs3804099), there was significant difference between the GC group and the control group in both genotype and allelic frequencies (χ²=5.617, P=0.018;χ²=6.467, P=0.011). The C allele and C allele carriers frequencies of gastric carcinoma group were significantly higher than those of controls (χ²=6.467, P=0.011;χ²=4.444, P=0.035). Compared with the wild type TT, the CC genotype could increase the risk of gastric cancer (OR=3.554, 95%CI=1.179-10.715). As for the TLR2 gene (rs38040100), there were no significant differences between the GC group and the control group in genotype, C allelic frequency and C allele carrier frequency (P>0.05). In all the indicators, no polymorphism was found to be related to EBVaGC in the studied population (P>0.05). CONCLUSION:There was an association between TLR2 rs3804099 gene polymorphism and gastric carcinoma, and the C allele may be a risk factor for gastric carcinoma. Carrying the C allele may increase the risk of gastric carcinoma. No association was observed between TLR2 (rs3804099 and rs38040100) gene polymorphism and EBVaGC.

Key words: gastric carcinoma, Epstein-Barr virus, Toll-like receptor, single nucleotide polymorphism

CLC Number:

R735.2

WEI Meirong, LIU Song, ZHAO Zhenzhen, WANG Xiaofeng, LUO Bing. TLR2 gene polymorphism and susceptibility to gastric carcinoma and EBV-associated gastric carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 341-346.

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TLR2 gene polymorphism and susceptibility to gastric carcinoma and EBV-associated gastric carcinoma

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