Carcinogenesis, Teratogenesis & Mutagenesis ›› 2015, Vol. 27 ›› Issue (3): 207-210,215.doi: 10.3969/j.issn.1004-616x.2015.03.010

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Assessment of DNA damage in different sub-groups of electronics industry workers by micro-whole blood comet assay

OU Xiaoyan1,2, LIU Xinxia3, JIANG Jun1,2, LU Yao1,2, ZHAO Zhiqiang1,2, FENG Jianqing3, SUN Yi1,2, YANG Yarui1,2, CUI Dong1,2, CHEN Jingli1,2, SHEN Biling1,2, XING Xiumei1,2, HE Yun1,2   

  1. 1. School of Public Health, Sun Yat-sen University, Guangzhou 510080;
    2. Key Laboratory of Environmental Pollution and Risk Assessment, Guangzhou 510080;
    3. Prevention and Control Center for Occupational Diseases, Zhongshan Center for Disease Control and Prevention, Zhongshan 528400, Guangdong, China
  • Received:2015-03-11 Revised:2015-04-16 Online:2015-05-30 Published:2015-05-30

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Abstract:

OBJECTIVE: To evaluate the DNA damage of different sub-groups of workers in the electronics industry, and to set up major preventive measures. METHODS:The 563 workers of an electronics factory in the Pearl River Delta Region were studied, according to their jobs we divided them into 5 groups, including lead exposure group (n167), isopropyl alcohol group (n287), noise group (n39), lead+high temperature group (n34), and isopro-panol+high temperature group (n36), with 90 pre-employment workers as controls. DNA damage was detected using micro-whole blood specimen by the comet assay method. RESULTS:Nuclear DNA content of lead+high temperature group was lower than the control group (P<0.05), and its tail DNA content, tail length, and tail moment were higher than the control group (P<0.05). The nuclear DNA content of isopropyl alcohol+high temperature group was lower than the control group (P<0.05), the comet tail DNA content, tail length, comet length, olive tail moment and tail moment were higher than the control group (P<0.05). CONCLUSION:Workers of different types in electronics industry chosen in this study had obvious DNA damage, especially isopropanol+high temperature group, followed by lead+high temperature group, to which special attention should be paid to these.

Key words: comet assay, blood, occupational groups, DNA damage

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