乙肝病毒S蛋白对人精子氧化应激的影响

doi:10.3969/j.issn.1004-616x.2015.03.009

Abstract

Abstract:

OBJECTIVE: It has been demonstrated that HBV is able not only to pass through the blood-testis barrier and integrate into sperm genome, leading to increase of the instability of sperm chromosomes, resulting in a various types of chromosomal aberrations, but also to destroy mitochondrial functions and induce loss of mitochondrial membrane potential (MMP), causing low sperm motility and reducing fertilization rate and fertilizing index. However, the exact pathogenic mechanism of such events is largely unknown so far. The purpose of this study is to explore the relationship between hepatitis B virus S protein (HBs) and oxidative stress in human sperm cells. METHODS:After the co-incubation of sperm cells with (0, 25, 50, 100 μg/mL) HBs, the reactive oxygen species (ROS) production was assessed by flow cytometric analyses;the total antioxidant capacity (TAC) level and the Aldetect (MDA-specific) lipid peroxidation (LP) level of the human sperm were measured by microplatereader. RESULTS:After incubation with 25 μg/mL of HBs for 3 h, the average rates of ROS-positive cells was significantly increased in the test groups as compared to those in the control groups, while TAC level was decreased when compared with the control. There was significantly higher level of MDA in the sperm cells exposed to 50 μg/mL of HBs for 3 h than that in the controls (P<0.05 or 0.01). HBs increased the MDA levels and the numbers of ROS-positive cells in a dose-dependent manner. HBs decreased the TAC levels in sperm cells in a dose-dependent manner. CONCLUSION:HBs exposure could lead to ROS generation, lipid peroxidation, TAC reduction, resulting in loss of sperm membrane integrity and causing sperm dysfunctions.

Key words: hepatitis B virus S protein, oxidative stress, reactive oxygen species, malondialdehyde

CLC Number:

R394.6

KANG Xiangjin, DING Yue, YANG Jie, DU Hongzi, LIU Jianqiao, HUANG Tianhua. Effects of hepatitis B virus S protein exposure on human sperm oxidative stress[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 202-206.

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Effects of hepatitis B virus S protein exposure on human sperm oxidative stress

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