不同还原态叶酸和胆碱组合对人结肠腺癌细胞hMLH1和hMSH2转录水平的影响

doi:10.3969/j.issn.1004-616x.2015.03.008

Abstract

Abstract:

OBJECTIVE: To explore the effects of folate and choline on human DNA mismatch repair genes hMLH1, hMSH2 expressions in human colonic adenocarcinoma cells. METHODS:Modified RPMI-1640 with the combinations of 12 μmol/L choline chlorine (CC) and 15, 30, 120 nmol/L of folic acid (FA) or 5-methyltetrahydrofolate (5-MeTHF), FA 120 nmol/L and CC (1.5, 3 μmol/L) were set as the intervening culture medium. COLO205 cells were cultured for 20 days. The transcription levels of hMLH1 and hMSH2 were analyzed by real-time fluorescence quantitative PCR. RESULTS:Transcription levels of hMLH1 and hMSH2 were significantly negatively correlated significantly with the concentrations of FA, 5-MeTHF or CC in tested cells (P<0.01). 5-MeTHF was more effective on hMLH1 and hMSH2 transcription regulation than same concentration of FA at 30 and 120 nmol/L (P<0.01 or 0.05). CONCLUSION:We concluded that FA, 5-MeTHF and CC deficiency up-regulated the expressions of hMLH1 and hMSH2. This may be the cellular response to DNA damage or abnormal methylation induced by folate and choline deficiency.

Key words: folic acid, 5-methyltetrahydrofolate, choline, hMLH1, hMSH2

CLC Number:

R151

LI Zhen, NI Juan, ZHOU Tao, WANG Han, WANG Xu. Effects of combining different reduced states of folate and choline on transcription of hMLH1 and hMSH2 in human colon adenocarcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 197-201,206.

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Effects of combining different reduced states of folate and choline on transcription of hMLH1 and hMSH2 in human colon adenocarcinoma cells

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