亲环素A过表达对高温、紫外线和顺铂引起的H1299细胞凋亡的影响

doi:10.3969/j.issn.1004-616x.2015.03.004

Abstract

Abstract:

OBJECTIVE: This study aimed to explore the effects of cyclophilin A (CyPA) over-expression in human lung adenocarcinoma cell line H1299 after treatments of high temperature, ultraviolet (UV) and cisplatin-induced apoptosis. METHODS:We set up H1299 CyPA over-expression cell lines via lentiviral transfection and used fluorescence microscope and Western blot analysis to analyze the expression of the target gene. H1299 and H1299 CyPA OE cell lines were cultured and exposed to treatments of high temperature, UV and cisplatin. High intension analysis platform was used to detect apoptosis of the two cell lines. RESULTS:Green fluorescent could be detected in H1299 CyPA OE cell line, and the protein CyPA expression was much higher than H1299 cell line, indicating that H1299 CyPA OE cell line was set up successfully. The apoptosis rate of H1299 CyPA OE cell line was much lower than H1299 cell line under different concentrations of cisplatin(P<0.05), but meanwhile the difference of apoptosis rates of H1299 CyPA OE cell line and H1299 cell lines showed no statistical significance(P>0.05). CONCLUSION:The over-expression of CyPA could confer resistance to apoptosis induced by cisplatin. CyPA may act as a potential gene target and provide new strategies of combined chemotherapy for lung cancer.

Key words: cyclophilin A, apoptosis, cisplatin, H1299 cell, lung cancer

CLC Number:

R730.5

YAN Jin, ZHAO Xiaoting, JIANG Mei, GU Meng, YUE Wentao. High temperature, ultraviolet light and cisplatin caused apoptosis of H1299 cell line which over expressed cyclophilin A[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 177-181,186.

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High temperature, ultraviolet light and cisplatin caused apoptosis of H1299 cell line which over expressed cyclophilin A

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