AMPK活性对HeLa细胞内质网功能稳态的影响

doi:10.3969/j.issn.1004-616x.2015.03.003

Abstract

Abstract:

OBJECTIVE: To observe the effects of AMPK activation on intracellular endoplasmic reticulum stress (ER stress) in Hela cells. METHODS:Cultured HeLa cells were divided into groups:1‰ DMSO control, tunicamycin (2 μg/mL) or MG132 (500 nmol/L), tunicamycin or MG132+Compound C (0.5 mmol/L);tunicamycin or MG132+ AICAR(2 mmol/L) and tunicamycin or MG132+metformin (2 mmol/L). AMPK activation and markers of ER stress were determined by Western blot, and then intracellular Ca²⁺ concentration was measured in HeLa cells by Indo-1 ratiometric Ca²⁺ analysis. RESULTS:Our results showed that tunicamycin and MG132 could increase expression of ER stress. Activation of AMPK by AICAR and metformin could reduce the expression of above markers, and decrease the concentration of intracellular Ca²⁺. CONCLUSION:AMPK activation could relieve intracellular ER stress, maintain the homeostasis of intracellular Ca²⁺ concentration, and further, may contribute to cellular adaptation and resistance to stress stimuli.

Key words: AMPK, cancer cells, endoplasmic reticulum, stress

CLC Number:

R730.2

LIANG Bin, YUAN Song, LIU Yanmin, SONG Xuhong. Effects of AMP-activated protein kinase activation on homeostasis of endoplasmic reticulum function in HeLa cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(3): 172-176.

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Effects of AMP-activated protein kinase activation on homeostasis of endoplasmic reticulum function in HeLa cells

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