nm23-H1基因转染对肺癌L9981细胞黏附分子表达的影响

doi:10.3969/j.issn.1004-616x.2015.02.012

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2015, Vol. 27 ›› Issue (2): 134-136,141.doi: 10.3969/j.issn.1004-616x.2015.02.012

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Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981

ZHENG Haixia¹, CUI Yongyan², SHEN Donglan³, PENG An³, HE Yanling³

1. Department of Health Examination, Peking University Shenzhen Hospital, Shenzhen 518036;
2. Department of Plastic Surgery, Peking University Shenzhen Hospital, Shenzhen 518036;
3. Department of Oncology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China

Received:2014-06-04 Revised:2014-12-03 Online:2015-03-31 Published:2015-03-31
About author:10.3969/j.issn.1004-616x.2015.02.012

Abstract

Abstract:

OBJECTIVE:To study influences of nm23-H1 gene transfection on the expressions of adhesion molecules in lung cancer cell line L9981. METHODS:Cell adhesion experiment and Boyden room were used to evaluate the change of cell adhesion and invasion. Semi-RT-PCR and flow cytometry were used to detect the mRNA and protein expressions of E-cadherin, integrin β1 and integrin β3 betwteen transfected and non-transfected cell lines. RESULTS: nm23-H1 cell line in vitro adhesion and invasion were all down-regulated. mRNA of E-cadherin in the transfected cell line was stronger than that of non-transfected cell line, but mRNAs of integrin β1 and integrin β3 of transfected cell line were weaker than that of non-transfected cell line. Flow cytometry showed similar results as above. CONCLUSION: nm23-H1 could reverse lung cancer malignant phenotype. nm23-H1 could up-regulate E-cadherin expression, while down-regulate expressions of integrins β1 and β3, thus inhibit the metastasis of lung cancer cells.

Key words: nm23-H1, lung cancer cells, E-cadherin, integrins, neoplastic metastasis

CLC Number:

R730.2

ZHENG Haixia, CUI Yongyan, SHEN Donglan, PENG An, HE Yanling. Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 134-136,141.

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Influences of nm23-H1 gene transfection on expressions of adhesion molecules in lung cancer cell line L9981

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