5个膀胱癌相关基因片段的DNA拷贝数改变及其意义

doi:10.3969/j.issn.1004-616x.2015.02.003

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2015, Vol. 27 ›› Issue (2): 91-94,100.doi: 10.3969/j.issn.1004-616x.2015.02.003

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Bladder cancer- associated DNA copy number changes in 5 gene fragments and their significance

XI Hao¹, CHEN Huang¹, BAI Jingqiao¹, ZHENG Yang¹, SHOU Jianzhong², LIU Yu¹, CHENG Shujun¹, GAO Yanning¹

1. Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021;
2. Department of Urological Surgical Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China

Received:2015-01-12 Revised:2015-03-06 Online:2015-03-31 Published:2015-03-31
About author:10.3969/j.issn.1004-616x.2015.02.003

Abstract

Abstract:

OBJECTIVE:To explore whether the DNA copy number changes in 5 gene fragments are specific abnormalities for bladder cancer in Chinese patients. METHODS:Real-time PCR was conducted to gauge DNA copy number alteration of the 5 gene fragments among commercial genome DNA of peripheral blood leucocyte (PBL) derived from Caucasian, genome DNA of PBL from Chinese healthy volunteers, the bladder cancer patients and their corresponding tumor tissues. RESULTS:Comparing with the Caucasian PBL, all the gene fragments revealed copy number changes with decreased CEP63, increased FOSL2, GHR and PAQR6, in the PBL of Chinese healthy volunteers, except ZFAND3. Comparing with the PBL of Chinese healthy volunteers, in the bladder tumor tissue samples, the copy number of CEP63, GHR and PAQR6 increased (all P<0.05);whereas FOSL2 (P=0.41) and ZFAND32 (P=0.062) revealed no statistical difference (all P>0.05). Comparing with the PBL of Chinese healthy volunteers, the copy number of CEP63 and PAQR6 increased but FOSL2 and ZFAND3 decreased in the PBL of bladder cancer patients (all P<0.01). Comparing with the PBL of the bladder cancer patients, gain of copy number was observed with CEP63, FOSL2, GHR and ZFAND3 (all P<0.01), while PAQR6 (P=0.325) exhibited no statistical difference, in the corresponding tumor tissues. CONCLUSION:Copy number variation of CEP63, FOSL2, GHR and PAQR6 was found between Chinese (Han) and Caucasian. Copy number gain of CEP63 and GHR may be involved in carcinogenesis of bladder cancer;while gain of PAQR6 was possibly associated with the genetic susceptibility for this malignancy.

Key words: bladder cancer, DNA copy number, CEP63, FOSL2, GHR, PAQR6, ZFAND3

CLC Number:

R730.2

XI Hao, CHEN Huang, BAI Jingqiao, ZHENG Yang, SHOU Jianzhong, LIU Yu, CHENG Shujun, GAO Yanning. Bladder cancer- associated DNA copy number changes in 5 gene fragments and their significance[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 91-94,100.

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Bladder cancer- associated DNA copy number changes in 5 gene fragments and their significance

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