3,3'-二吲哚甲烷对过氧化氢诱导人角质形成细胞氧化应激的预防作用及其机制

doi:10.3969/j.issn.1004-616x.2015.02.001

Abstract

Abstract:

OBJECTIVE:To observe the effects of 3, 3'-diindolylmethane (DIM) on oxidative stress induced by hydrogen peroxide (H₂O₂) in HaCaT cells. METHODS:HaCaT cells were treated with different concentrations of H₂O₂ to establish the models of oxidative stress. CCK-8 assay was performed to evaluate the inhibitive effects of DIM (1-20 μmol/L) on cells proliferation. The intracellular reactive oxygen species (ROS) levels were measured with flow cytometry. Furthermore, the effects of DIM (0, 5 and 10 μmol/L) on H₂O₂-induced the phosphorylation level of nuclear factor κB (p-NF-κB), the expression of mitogen activated protein kinase (MAPKs) were detected with Western blot. RESULTS: The cell model of oxidative stress was successfully established. The results of CCK-8 assay showed that in the doses of 1-10 μmol/L, DIM did not have obvious influence on cell viability (P>0.05). Flow cytometry results indicated that pre-treatment with DIM (10 μmol/L) could inhibit the level of intracellular ROS (P<0.05). With increasing concentration of DIM, the levels of p-p38-MAPK, p-JNK and p-NF-κB were significantly depressed. CONCLUSION: DIM could protect HaCaT cells from H₂O₂-induced oxidative stress via suppressing production of ROS levels and down-regulating the expression of NF-κB and members of MAPKs. DIM might be used as an effective drug to treat or reduce oxidative stress-mediated injury in skin cells.

Key words: 3,3'-diindolylmethane, HaCaT cells, hydrogen peroxide, oxidative stress

CLC Number:

R730.2

YIN Hua, LI Wenxue, LI Mengcheng, DENG Yuting, LI Jinhui, LI Juntao, WU Jinyin, YANG Guangyu, LIN Zhongning, ZHU Wei. Preventive effect and mechanisms of 3,3'-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(2): 81-85,90.

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Preventive effect and mechanisms of 3,3'-diindolylmethane on oxidative stress induced by hydrogen peroxide in HaCaT cells

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