三氯乙烯对L-02肝细胞中SET相互作用蛋白eEF1A1、eEF1A2和DDB1 mRNA表达水平的影响

doi:10.3969/j.issn.1004-616x.2015.01.014

Abstract

Abstract:

OBJECTIVE: To research the effects of trichloroethylene on the RNA expressions of SET-associated proteins including eEF1A1，eEF1A2 and DDB1 in L-02 cells. METHODS：L-02 cells，being in the logarithmic growth phase，were treated with different concentrations (1.0，2.0，4.0，8.0 mmol/L) of trichloroethylene for 24 h， and DMSO were used as control. The mRNA levels of eEF1A1， eEF1A2 and DDB1 were analyzed with qPCR. RESULTS：The results showed that mRNA levels of DDB1 and eEF1A2 were significantly up-regulated under low concentration of trichloroethylene (1.0 mmol/L) and were significantly down-regulated with increasing concentrations of trichloroethylene. The mRNA levels of eEF1A1 were significantly up-regulated with increasing of trichloroethylene concentration. CONCLUSION：Exposure of trichloroethylene could alter mRNA expression of SET-associated proteins in L-02 cells.

Key words: trichloroethylene, cytotoxicity, SET-associated proteins, mRNA

HUANG Aibo，HONG Wenxu，YE Jinbo，LIU Jianjun，XU Hua. Effects of trichloroethylene on mRNA expression of SET-associated proteins including eEF1A1，eEF1A2 and DDB1 in L-02 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2015.01.014.

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Effects of trichloroethylene on mRNA expression of SET-associated proteins including eEF1A1，eEF1A2 and DDB1 in L-02 cells

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