乙肝病毒S蛋白诱导人精子凋亡的分子机制

doi:10.3969/j.issn.1004-616x.2015.01.010

Abstract

Abstract:

OBJECTIVE: To explore the mechanisms of human sperm apoptosis by hepatitis B virus S protein. METHODS：After the co-incubation of sperms with 0，25，50 and 100 μg/mL HBS for 3 h，the externalization of membrane phosphatidylserine (PS)；Caspase-3，-8，-9 activation and DNA fragmentation of human sperm were assessed. RESULTS：After incubation，the average rates of Annexin V–positive/propidium iodide (PI)-negative sperm，Caspase-3，-8，-9 positive sperm and TUNEL-positive sperm were significantly increased in the test groups as compared to those in the control groups (P<0.05 or P<0.01)，and in a dose-dependent manner. CONCLUSION：HBs exposure could lead to PS externalization，activation of caspases，and DNA fragmentation，resulting in increased apoptosis of sperms and causing sperm dysfunction.

Key words: HBs, apoptosis, membrane with normal integrity, sperm function

KANG Xiangjin，DING Yue，YANG Jie，DU Hongzi，LIU Jianqiao，HUANG Tianhua. Study of the mechanisms of human sperm apoptosis by hepatitis B virus S protein[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2015.01.010.

References

[1] Bruss V，Gerhardt E，Vieluf K，et al. Functions of the large hepatitis B virus surface protein in viral particle morphogenesis [J]. Intervirology，1996，39(1)：23-31.

[2] Zhou XL，Sun PN，Huang TH，et al. Effects of hepatitis B virus S protein on human sperm function[J]. Hum Reprod， 2009，24(7)：1575-1583.

[3] Moretti E，Federico MG，Giannerini V，et al. Sperm ultrastructure and meiotic segregation in a group of patients with chronic hepatitis B and C[J]. Andrologia，2008，40(3)：286-291.

[4] Singh NP，Muller CH，Berger RE. Effects of age on DNA double-strand breaks and apoptosis in human sperm[J]. Fertil Steril，2003，80(6)：1420-1430.

[5] 胡静熠. 生精过程中细胞凋亡的基因和激素调控机制[J]. 国外医学：卫生学分册，2001，28(2)：69-74.

[6] Glander HJ，Schaller J. Binding of annexin V to plasma membranes of human spermatozoa：a rapid assay for detection of membrane changes after cryostorage[J]. Mol Hum Reprod， 1999，5(2)：109-115.

[7] Weng SL，Taylor SL，Morshedi M，et al. Caspase activity and apoptotic markers in ejaculated human sperm[J]. Mol Hum Reprod，2002，8(11)：984-991.

[8] Paasch U，Grunewald S，Agarwal A. Activation pattern of caspases in human spermatozoa[J]. Fertil Steril，2004，81 (Suppl 1)：802-809.

[9] Bellamy CO，Malcomson RD，Harrison DJ，et al. Cell death in health and disease：the biology and regulation of apoptosis[J]. Semin Cancer Biol，1995，6(1)：3-16.

[10] Richburg JH. The relevance of spontaneous- and chemically-induced alterations in testicular germ cell apoptosis to toxicology[J]. Toxicol Lett，2000(112/113)：79-86.

[11] Shen HM，Dai J，Chia SE，et al. Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality[J]. Hum Reprod，2002，17(5)：1266-1273.

[12] Hadchouel M，Scotto J，Huret JL，et al. Presence of HBV DNA in spermatozoa：a possible vertical transmission of HBV via the germ line[J]. J Med Virol，1985，16(1)：61-66.

[13] Glander HJ，Schaller J. Binding of annexin V to plasma membranes of human spermatozoa：a rapid assay for detection of membranechanges after cryostorage[J]. Mol Hum Reprod， 1999，5(2)：109-115.

[14] Marchenko ND，Zaika A，Moll UM. Death signal-induced localization of p53 protein to mitochondria. A potential role in apoptotic signaling[J]. J Biol Chem，2000，275(21)：16202-16212.

[15] Almeida C，Correia S，Rocha E，et al. Caspase signalling pathways in human spermatogenesis[J]. J Assist Reprod Genet，2013，30(4)：487-495.

Study of the mechanisms of human sperm apoptosis by hepatitis B virus S protein

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Boru, QIN Shuangjian, GUAN Lan, LI Runbing, CAI Ying, PU Jiening, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 on oxidative damage and apoptosis in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 360-364.
[2]	HUANG Xiaohua, NIU Yongdong, SHI Ganggang. Inhibitory effects and mechanisms of nuclear receptor FXR activation in cervical cancer cell line CaSki [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 302-306.
[3]	WANG Xikai, MENG Qinghe, GAO Yanlu. miR-223-3p negatively regulated ECT2 to induce apoptosis in non-small cell lung cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 187-192.
[4]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, ZENG Ming, XIAO Fang, XU Xinyun. Effects of p38MAPK gene on PM_2.5-induced expression of oncogenes and apoptosis genes in HK-2 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 129-135,142.
[5]	MA Shunpeng, WANG Quankai, WANG Miao, SONG Jiayang, WUHAN Baolier, XIE Guangyun, XU Jianning. Long non-coding RNA regulates apoptosis during glycidyl methacrylate-induced malignant transformation of 16HBE cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 1-5,31.
[6]	LI Boru, QIN Shuangjian, LI Runbing, CAI Ying, ZHENG Kai, WANG Bingyu, ZENG Ming, XIAO Fang, XU Xinyun. Effects of PM_2.5 exposure on expression of oncogenes and apoptosis genes in human renal epithelial cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 6-11.
[7]	HUANG Lili, ZHANG Mengyun, LIU Keliang, PANG Dingguo, LIU Lida, XU Peiyu. Hepatotoxicity of 2,4-dichlorophenoxyacetic acid in young SD rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 22-27.
[8]	CUI Zhaoyang, LI Suna, JIANG Xuqian, WANG Yidan, HOU Liying. Activation of the TRAIL death receptor by apigenin for induction of apoptosis in the gastric cancer SGC-7901 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 32-36.
[9]	LAN Li, CHEN Haili, XU Lei. Mechanism of RBMS3-S3 on the proliferation, apoptosis and invasion of cervical cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 438-443.
[10]	LIU Peng, BAI Yixiao. Effect of miR-29a on proliferation and migration of human gastric cancer cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 452-456,463.
[11]	YANG Xingxiao, ZHANG Xueyuan, ZOU Naiyi, SHAN Bao'en, MA Ming, ZHU Shuchai. Effect of HMGB1 silencing by siRNA on radiosensitivity of esophageal carcinoma KYSE30 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 269-274.
[12]	QIN Shuangjian, LI Boru, CAI Ying, ZHENG Kai, WANG Bingyu, LI Runbing, XIAO Fang, ZENG Ming, XU Xinyun. PM_2.5 exposure on expression of oncogenes and apoptosis genes in hepatocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 281-285.
[13]	CAI Ying, ZHENG Kai, QIN Shuangjian, LI Boru, YU Shuyuan, LIU Ning, JI Jiajia, ZHANG Zhaohui, XU Xinyun. Silencing of c-fos gene on expression of oncogenes and apoptosis genes in HBE cells exposed to PM_2.5 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(4): 298-303,308.
[14]	HUA Chenfeng, ZHAO Junwei, SHANG Pingping, FAN Meijuan, XIE Fuwei, LI Xiang. Induction of cytotoxicity, inflammatory factor release and apoptosis by cigarettes with different tar releases [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 182-186.
[15]	WANG Yaojie, XIANG Xiaohan, HAN Lina, LI Jun, HUO Bingjie, ZHAO Lianmei. Inhibitory effects of ethanol extract from Euphorbia humifusa on gastric cancer cells in vitro [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 187-193.