Establishment of flow cytometric in in vitro micronucleus assay
OU Hongmei,ZHOU Changhui,TU Honggang,HUANG Pengcheng,CHANG Yan*
China State Institute of Pharmaceutical Industry, Shanghai Institute of Pharmaceutical Industry, National Shanghai Center for New Drug Safety Evaluation & Research, Shanghai 201203, China
[1] Corvi R,Albertini S,Hartung T,et al. ECVAM retrospective validation of in vitro micronucleus test (MNT)[J]. Mutagenesis, 2008,23(4):271-283.[2] OECD. Guidlines for Testing of Chemicals. In vitro mammalian cell micronucleus test (MNvit)[EB/OL]. [2013-05-20] http:// www. oecd.org/env/test guidelines. html.[3] ICH. International Conference on Harmonization. Guidance on genotoxicity testing and data interpretation for pharmaceuticals intended for human use S2 (R1)[EB/OL]. [2013-05-20]http: //www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Safety/S2R1.html.[4] Bryce SM,Bemis JC,Mereness JA,et al. Interpreting In vitro micronucleus positive results:simple biomarker matrix discriminates clastogens,aneugens,and misleading positive agents[J]. Environ Mol Mutagen,2014. doi:10.1002/em.21868.[5] Tilmant K,Gerets HH,De Ron P,et al. The automated micronucleus assay for early assessment of genotoxicity in drug discovery[J]. Mutat Res,2013,751(1):1-11.[6] Bull CF,Beetstra-Hill S,Benassi-Evans BJ,et al. Application and adaptation of the in vitro micronucleus assay for the assessment of nutritional requirements of cells for DNA damage prevention[J]. Mutagenesis,2011,26(1):193-197.[7] Bryce SM,Avlasevich SL,Bemis JC,et al. Flow cytometric 96-well microplate-based in vitro micronucleus assay with human TK6 cells:protocol optimization and transferability assessment[J]. Environ Mol Mutagen,2013,54(3):180-194.[8] Yao J,Gao Q,Mi Q,et al. In vitro micronucleus assay for the analysis of total particulate matter in cigarette smoke: Comparison of flow cytometry and laser scanning cytometry with microscopy[J]. Mutat Res,2013,755(2):120-125.[9] Avlasevich SL,Bryce SM,Cairns SE,et al. In vitro micronucleus scoring by flow cytometry:differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability[J]. Environ Mol Mutagen,2006,47(1): 56-66.[10] Shi J,Bezabhie R,Szkudlinska A. Further evaluation of a flow cytometric in vitro micronucleus assay in CHO-K1 cells:a reliable platform that detects micronuclei and discriminates apoptotic bodies[J]. Mutagenesis,2010,25(1):33-40.[11] Sobol Z,Homiski ML,Dickinson DA,et al. Development and validation of an in vitro micronucleus assay platform in TK6 cells [J]. Mutat Res,2012,746(1):29-34.[12] Bryce SM,Shi J,Nicolette J,et al. High content flow cytometric micronucleus scoring method is applicable to attachment cell lines[J]. Environ Mol Mutagen,2010, 51(3): 260-266.[13] Bryce SM,?Bemis JC,?Mereness JA,et al. Interpreting in vitro micronucleus positive results:simple biomarker matrix discriminates clastogens,aneugens,and misleading positive agents[J]. Environ Mol Mutagen,2014,55(7):542-555.[14] Fowler P,?Smith R,?Smith K,et al. Reduction of mis-leading (“false”) positive results in mammalian cell genotoxicity assays. III:Sensitivity of human cell types to known genotoxic agents[J]. Mutat Res Genet Toxicol Environ Mutagen,2014,767:28-36.[15] Yuki K,Ikeda N,Nishiyama N,et al. The reconstructed skin micronucleus assay in EpiDerm?:Reduction of false-positive results-a mechanistic study with epigallocatech in gallate[J]. Mutat Res,2013,757(2):148-157.[16] Jetten MJ,Kleinjans JC,Claessen SM,et al. Baseline and genotoxic compound induced gene expression profiles in HepG2 and HepaRG compared to primary human hepatocytes[J]. Toxicol In Vitro,2013,27(7):2031-2040.[17] Sun B,Ross SM,Trask OJ,et al. Assessing dose-dependent differences in DNA-damage,p53 response and genotoxicity for quercetin and curcumin[J]. Toxicol In Vitro,2013,27(6): 1877-1887.