大豆苷元对乳腺癌生长和血管生成的影响

doi:10.3969/j.issn.1004-616x.2015.01.004

Abstract

Abstract:

OBJECTIVE: To evaluate the inhibitory effects of daidzein on angiogenesis of breast carcinoma，and to study their anti-cancer mechanisms by using the model of 7，12-dimethylbenz[α]anthraxcene (DMBA)-induced breast carcinoma in SD rats. METHODS：The DMBA -treated SD rats were randomly divided into control，10，25，50 and 75 mg/kg daidzein-gavaged groups. The growth of tumors was assessed in each group. The tumor microvessel density (MVD) was determined，and tumor proliferation index and apoptosis index were calculated. Moreover，vascular endothelial growth factor (VEGF)，basic fibroblast growth factor (bFGF) and endostatin levels were measured by ELISA. RESULTS：Compared with the control group，tumor mass was reduced more significantly in the 50 and 75 mg/kg daidzein groups (P<0.05). The apoptosis index of 75 mg/kg daidzein group was increased，and the effect of inhibiting tumor angiogenesis was significant (all P<0.05). Compared with the control group，the VEGF levels of 25，50 and 75 mg/kg daidzein groups were all decreased，in a dose-dependent manner(P<0.05)，meanwhile endostatin levels were increased in the 50 and 75 mg/kg daidzein groups，and the differences were statistically significant (all P<0.05). CONCLUSION： Higher dose of daidzein (≥50 mg/kg) could decrease MVD of mammary tumor and inhibit the growth of breast carcinoma effectively in rats.

Key words: breast carcinoma, daidzein, microvessel density, vascular endothelial growth factor, endostatin

KANG Xinmei，WANG Hui，WANG Li，TANG Dabei，ZHANG Qingyuan. Effect of daidzein on the growth and angiogenesis of breast carcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2015.01.004.

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Effect of daidzein on the growth and angiogenesis of breast carcinoma

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