洋地黄毒苷对肺癌NCI-H446与A549细胞增殖和周期的影响

doi:10.3969/j.issn.1004-616x.2015.01.002

Abstract

Abstract:

OBJECTIVE: To investigate the effects of digitoxin on proliferation and cell cycle of human lung cancer cell lines NCI-H446 and A549，and to explore its possible mechanisms. METHODS：NCI-H446 and A549 cells were treated with digitoxin at different concentrations(10，20，40，80 and 160 nmol/L). The effect on proliferation of NCI-H446 and A549 cells were detected by MTT assay. Flow cytometry was used to test the cell cycle distribution of NCI-H446 and A549 cells. Protein expressions of Cyclin A1 and P21 in NCI-H446 and A549 cells were evaluated by Western blot. RESULTS：As compared with control group，cell proliferation was inhibited by digitoxin in a dose- and time-dependent manner(P＜0.05)，the IC50 value for digotoxin were 61.26 nmol/L in NCI-H446 and 110.73 nmol/L in A549 at 48 h. After 48 h treatment，the proportion of NCI-H446 and A549 cells in G0/G1 phase was decreased，while the proportion in S phase was increased. Western blot analysis showed that digitoxin could significantly up-regulate the expression of Cyclin A1 and down-regulate the expression of P21 in a dose-dependent way(P<0.05). CONCLUSION： Digitoxin could exert an the anti-proliferative effect on NCI-H446 and A549 cells and induce S phase arrest in vitro. The mechanism may be related to the expressions of proteins associated with cell cycle.

Key words: digitoxin, lung cancer, regulation of cell cycle, Cyclin A1

LIU Feng，CHEN Zhitian，SHAO Kongjian，SA Benzhong. Effect of digitoxin on proliferation and cell cycle of NCI-H446 and A549 cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2015.01.002.

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Effect of digitoxin on proliferation and cell cycle of NCI-H446 and A549 cells

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