纳米硫化镉与硫化镉雄性生殖毒性及相关机制的研究

doi:10.3969/j.issn.1004-616x.2014.06.009

Abstract

Abstract:

OBJECTIVE: To compare the effects of normal cadmium sulfide (CdS) and Nano-CdS on the male reproductive system of mice，and to investigate the mechanisms of reproductive toxicity. METHODS：36 male ICR mice were randomly divided into control group，and two experimental groups which were exposed to 50 mg/kg Nano-CdS and CdS，respectively. Mice in control group were exposed to same volume of physiological saline . After 45 days，levels of cadmium accumulation in testis were determined directly by GF AAS. The rate of sperm abnormality was also calculated. Serum testosterone levels were measured by enzyme-linked immunosorbent assay (ELISA)，expression levels of P450scc and P450-17α mRNA were determined by real-time PCR. RESULTS：Contents of cadmium in Nano-CdS and CdS groups were (425.46±73.89) and (183.59±32.46) ng/g，respectively，and were higher than the control (80.18±13.29) (P<0.05). Sperm deformity rate in CdS group (2.28%) was significantly higher than the control (1.48%) (P<0.05)，Nano-CdS group (1.73%) was also increased. Concentrations of testosterone in CdS and Nano-CdS groups were(12.31±1.10) and (15.88±5.41) ng/dL，respectively，and were significantly lower than control group (68.41±11.36) ng/dL (P<0.05). Expression levels of P450scc mRNA in Nano-CdS and CdS groups were (0.50±0.11) and (0.84±0.48)，respectively， expression levels of P450-17α mRNA were (0.51±0.13) and (0.72±0.06)，respectively. Except P450scc mRNA in CdS group，the mRNA expression levels in experimental groups were significantly lower than control group (P<0.05). CONCLUSION：Content of cadmium accumulation in testis and sperm deformity rate were increased by exposure to Nano-CdS and CdS，with a significant reduction in serum testosterone levels and expression levels of P450scc and P450-17α mRNA.

Key words: cadmiumsulfidenano-particles, sperm , abnormality, testosterone, cytochrome , P450, side-chain , cleavage , enzyme, steroid , 17-alpha-hydroxylase

ZHOU Qing-hong，JIANG Shu-qing，LIU Ying-hua，ZHANG Li，ZHAO Chun-hong，WANG Xiao-jun，ZHANG Da-long，ZHANG Jing-shu. Study on the toxicity and related machanisms of Nano-CdS and CdS on male reproduction[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.06.009.

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Study on the toxicity and related machanisms of Nano-CdS and CdS on male reproduction

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