纳米银对中国仓鼠肺成纤维细胞的细胞毒性和遗传毒性

doi:10.3969/j.issn.1004-616x.2014.06.006

Abstract

Abstract:

OBJECTIVE: To study the cyto-and genotoxicities of silver nanoparticles (silver-NPs) on Chinese hamster lung fibroblast cell line (CHL) and explore their possible mechanisms. METHODS：CHLs were exposed to gradient concentrations of silver-NPs 2.5，5.0，10.0，20.0，40.0 and 80.0 μg/mL，then MTT assay was employed to reveal the IC50 which was used to evaluate the cytotoxicity potential of silver-NPs. Cell cycle analysis was performed with PI dye，and proliferation indexes were calculated as well. Apoptosis was detected with Annexin V FITC- PI dye，morphology changes after exposure and forward scatter channel (FSC) and side scatter channel(SSC) were recorded simultaneously. Chromosomal aberrations were analyzed by reading slides with metaphases stained by Giemsa. RESULTS：Cytotoxicity on CHL was positively associated with the concentration of silver-NPs (r=0.804，P<0.05)， IC50 was 21.68 μg/mL. Morphology changes were clearly visible after CHLs were treated with 22.0 μg/mL silver-NPs for 24 h. Significantly enhanced SSC signal existed in CHL populations after periods of exposure to silver-NPs，which implied uptake of silver-NPs. CHL cycle was found to be arrested in G2/M phase，and cell proliferation index as well as G0/G1 phase，S phase were significantly changed compared with vehicle control (P<0.01). Furthermore，apoptosis was found to be significantly increased after 22.0 μg/mL silver-NPs exposure for 24 h (P<0.01). Silver-NPs exposure also induced CHL chromosome structure aberration with more than 5% incidence at all tested concentrations (P<0.01). Tetraploid in high and middle concentrations- treated groups were 10 times higher than the vehicle control(P<0.01). CONCLUSION：Silver-NPs showed significant cytotoxicity and genotoxicity in CHL，where mitochondrial dysfunction and apoptosis might play important roles.

Key words: silver , nanoparticle, Chinese , hamster , lung , fibroblast , cell, cytotoxicity, genotoxicity, cell , cycle, apoptosis

YU Yong-sheng，SHI Chang，XU Li-ming，HAN Gang，YIN Ji-ye，MA Hua-zhi,DING Ri-gao. Cytotoxicity and genotoxicity of silver nanoparticles on the Chinese hamster lung fibroblast cell line[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.06.006.

References

[1] Rai M，Yadav A，Gade A. Silver nanoparticles as a new generation of antimicrobials[J]. Biotechnol Adv，2009，27(1)： 76-83.

[2] Edwards-Jones V. The benefits of silver in hygiene，personal care and healthcare[J]. Lett Appl Microbiol，2009，49(2)： 147-152.

[3] Ahamed M，Alsalhi MS，Siddiqui MKJ. Silver nanoparticle applications and human health[J]. Clin Chim Acta，2010，411 (23/24)：1841-1848.

[4] Li XF，Xu LM，Shao AL，et al. Cytotoxic and genotoxic effects of silver nanoparticles on primary Syrian hamster embryo (SHE) cells[J]. J Nanosci Nanotechno，2013，13(1)：161-170.

[5] Ghosh M，Manivannan J，Sinha S，et al. In vitro and in vivo genotoxicity of silver nanoparticles[J]. Mutat Res-Gen Tox En， 2012，749(1/2)：60-69.

[6] Unfried K，Albrecht C，Klotz LO，et al. Cellular responses to nanoparticles：Target structures and mechanisms[J]. Nano-toxicology， 2007，1(1)：52-71.

[7] Singh RP，Ramarao P. Cellular uptake，intracellular trafficking and cytotoxicity of silver nanoparticles[J]. Toxicol Lett，2012，213(2)：249-259.

[8] Beer C，Foldbjerg R，Hayashi Y，et al. Toxicity of silver nanoparticles-nanoparticle or silver ion?[J]. Toxicol Lett， 2012，208(3)：286-292.

[9] Asha-Rani PV，Mun GLK，Hande MP，et al. Cytotoxicity and genotoxicity of silver nanoparticles in human cells[J]. Acs Nano，2009，3(2)：279-290.

[10] Kim HR，Kim MJ，Lee SY，et al. Genotoxic effects of silver nanoparticles stimulated by oxidative stress in human normal bronchial epithelial (BEAS-2B) cells[J]. Mutat Res-Gen Tox En，2011，726(2)：129-135.

[11] Jahnen-Dechent W，Simon U. Function follows form：shape complementarity and nanoparticle toxicity[J]. Nanomedicine， 2008，3(5)：601-603.

[12] 李平，周元国. ATM和ATR：在G2/M期阻滞与凋亡中的作用及相互关系[J]. 国外医学：分子生物学分册，2003， 25(3)：159-162.

[13] Chao DT，Korsmeyer SJ. Bcl-2 family：Regulators of cell death[J]. Annu Rev Immunol，1998，16(1)：395-419.

[14] Gopinath P，Gogoi SK，Sanpui P，et al. Signaling gene cascade in silver nanoparticle induced apoptosis[J]. Colloids Surf B Biointerfaces，2010，77(2)：240-245.

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Cytotoxicity and genotoxicity of silver nanoparticles on the Chinese hamster lung fibroblast cell line

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