LncRNA-UCA1与淮安食管癌发病风险的初步研究

doi:10.3969/j.issn.1004-616x.2014.06.002

Abstract

Abstract:

OBJECTIVE: To analyze LncRNA differential expression in tumoral and normal-paired tissues of esophageal squamous cell carcinoma in Huaian and to explore the role of tumor-associated LncRNAs in the pathogenesis of esophageal squamous cell carcinoma. METHODS：Total RNA was extracted from 3 patients' esophageal cancer tissues and corresponding adjacent normal tissues. Then，each sample was amplified and transcribed into fluorescent cRNA with a random priming method. The labeled cDNAs were hybridized onto the Human LncRNA Array v2.0 (8×60K，Arraystar). After being scanned，Agilent GeneSpring GX v11.5.1 was used to screen the differentially expressed LncRNAs. qRT-PCR was used to analyze LncRNA expression in cells and tissues of esophageal cancer. RESULTS：Among the 1 019 significantly differentially expressed LncRNAs (Fold Change≥2.0，P≤0.05)，there were 399 LncRNAs overexpressed and 620 under- expressed. GO analysis and Pathway analysis showed that the differentially expressed mRNAs，regulated by LncRNAs，were mainly associated with the cell cycle，cell differentiation， protease activities etc. Compared to LncRNA differences related to the Gibb's esophageal expression profile analysis，LncRNA-UCA1 might participate in esophageal cancer development. qRT-PCR results showed that LncRNA-UCA1 was significantly under-expressed in esophageal cancer cells and esophageal cancer tissues when compared with immortalized esophageal epithelial cell and adjacent normal tissues. CONCLUSION：A total of 1 019 significantly differentially expressed LncRNAs were identified，and LncRNA-UCA1 may be a potential biomarker in the early detection and diagnosis of esophageal cancer.

Key words: esophageal , carcinoma, LncRNA-UCA1, qRT-PCR, tumor , molecular , markers

LI Xia-jun，YANG Miao，DONG Zi-cheng，LIU Ran，YIN Li-hong，PU Yue-pu. Association between LncRNA-UCA1 expression and the risk for esophageal carcinoma in Huaian[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.06.002.

References

[1] Caley DP，Pink RC，Trujillano D，et al. Long noncoding RNAs，chromatin，and development[J]. Sci World J，2010， 8(10)：90-102.

[2] Gibb EA，Vucic EA，Enfield KS，et al. Human cancer long non-coding RNA transcriptomes[J]. PLoS One，2011， 6(10)：e25915.

[3] Mitra SA，Mitra AP，Triche TJ，et al. A central role for long non-coding RNA in cancer[J]. Genetics，2012，3(17)：1-9.

[4] Wilusz JE，Sunwoo H，Spector DL，Long noncoding RNAs： functional surprises from the RNA world[J]. Genes Dev，2009， 23(13)：1494-1504.

[5] Wang Y，Chen W，Yang C，et al. Long non-coding RNA UCA1a (CUDR) promotes proliferation and tumorigenesis of bladder cancer[J]. Int J Oncol，2012，41(1)：276-284.

[6] Wang F，Li X，Xie XJ，et al. UCA1，a non-protein-coding RNA up-regulated in bladder carcinoma and embryo， influencing cell growth and promoting invasion[J]. FEBS letters，2008，582(13)：1919-1927.

[7] Yang C，Li X，Wang Y，et al. Long non-coding RNA UCA1 regulated cell cycle distribution via CREB through PI3-K dependent pathway in bladder carcinoma cells[J]. Gene， 2012，496(1)：8-16.

[8] Fan Y，Shen B，Tan M，et al. Long non-coding RNA UCA1 increases chemoresistance of bladder cancer cells by regulating Wnt signaling[J]. FEBS Journal，2014，281(7)：1750-1758.

[9] Huang J，Zhou N，Watabe K，et al. Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1)[J]. Cell Death Dis，2014，5(1)：1008-1018.

[10] 谢小娟，李旭，王帆，等．非编码RNA UCA1基因的细胞定位和组织表达谱分析[J]．南方医科大学学报，2010，30( 1) ：57-60.

[1]	ZHU Xinping, JIA Di, LIN Shaohui, LIU Chen, LI Jingyi, ZHAO Weiming. Inhibitory effects of a novel polyoxometalate drug {BiW₈O₃₀}on liver cancer SK-HEP-1 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 47-52,66.
[2]	GUO Zhaomeng, ZHANG Hua, ZHANG Peng. Mechanisms of Orai1 on invasion and epithelial-mesenchymal transformation in nasopharyngeal carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 420-425.
[3]	ZHANG Zongming. Does thyroid microcarcinoma require surgery [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 325-326.
[4]	YANG Xingxiao, ZHANG Xueyuan, ZOU Naiyi, SHAN Bao'en, MA Ming, ZHU Shuchai. Effects of HMGB1 silencing on migration, invasion and radiosensitivity of esophageal squamous cell carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 327-333.
[5]	YANG Liyan, LIU Zou, HAO Jiajie, ZHANG Yu, WANG Mingrong. Study on the mechanism of nucleo-plasma translocation of p62 protein in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 338-344.
[6]	JIAO Wenjing, ZHANG Jinyan, MA Ming, GUO Xiujuan, FENG Junhua, SHAO Junguo, JIAO Wenpeng. Expression and clinical significance of MTDH and FoxM1 in hepatocellular carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 349-353,359.
[7]	CHANG Chen, YANG Liyan, CAI Hongqing, YUAN Qing, HAO Jiajie, WANG Mingrong. Expressions and clinical implications of SERPINE1 mRNA in esophageal squamous carcinoma tissues [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 245-249.
[8]	ZENG Zhuoying, WU Desheng, LU Jingjing, LIAO Hui, LAI Hongpiao, YUAN Jianhui, HU Zhangli. Mediation by estrogen receptor α on oxidative phosphorylation pathways in development of lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 262-268.
[9]	ZHENG Junwen, CHANG Chen, HAO Jiajie, CAI Yan, WANG Mingrong, ZHANG Yu. Investigation on expression and function of NEDD4 in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 203-207,224.
[10]	JIAO Wenjing, GUO Xiujuan, SHAO Junguo, JIAO Wenpeng, ZHANG Jinyan. Relationships among blood cell ratios, clinicopathological characteristics and prognosis in esophageal squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 213-217.
[11]	ZHANG Die, LIU Xianghe, HUO Miaomiao, LIU Mei, XU Ningzhi, ZHU Hongxia. Effects of miR-195-5p on radiosensitivity of esophageal squamous cell carcinoma cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 81-88,94.
[12]	ZHANG Na, FAN Zhilu, YANG Liyan, CHANG Chen, CAI Yan, HAO Jiajie, WANG Mingrong. Clinical significance of decreased SERPINB5 expression in esophageal squamous cell carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(2): 101-109.
[13]	WANG Yaokun, CHEN Juexiao, ZHANG Mingyuan, SHAO Changli, YANG Yu, SHANG Yu. Database analysis of genome variations and targeted drug screening of anaplastic thyroid cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 53-57.
[14]	LI Jinglei, HOU Wei. ceRNA network-based stigmasterolintervention on lung squamous cell carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 423-429.
[15]	LIN Kaihuang, CAI Gaoyang, LIU Xincheng. Effect of mTOR inhibitor AZD2014 on proliferation of hepatoma HCC cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 448-451.

Association between LncRNA-UCA1 expression and the risk for esophageal carcinoma in Huaian

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments