邻苯二甲酸二(2-乙基己)酯对小鼠肺细胞DNA的损伤作用

doi:10.3969/j.issn.1004-616x.2014.05.015

Abstract

Abstract:

OBJECTIVE: To study DNA damage induced by di (2-ethylhexyl) phthalate (DEHP) to mice lung cells. METHODS：The negative control group was exposed to saline. Extracted mice lung cells were exposed to different concentrations of DEHP(5，25，125，625 μmol/L) and single cell gel electrophoresis was used to test the DNA breakage degree. RESULTS：Compared with the control group，tail DNA percentage，tail moment and Olive tail moment of 125 μmol/L and 625 μmol/L DEHP-treated groups were increased significantly (P＜0.05). CONCLUSION： DEHP could induce DNA breakage of mice lung cells in vitro.

Key words: di(2-ethylhexyl) phthalate, single cell gel electrophoresis, lung cells, DNA damage

WANG Hai-xue，FENG Fei-fei，GAO Liu-chuang，BA Yue，FAN Qing-tang，DUAN Li-ju*. DNA damage induced by di(2-ethylhexyl) phthalate in mice lung cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2014.05.015.

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DNA damage induced by di(2-ethylhexyl) phthalate in mice lung cells

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